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作 者:周丽萍[1] 马艳 杨锦亮 ZHOU LIping;MA Yan;YANG Jinliang(The General Hospital of Ningxia Medical University,Yinchuan,750004,China;The Hospital O Yinchuan Traditional Chinese Medicine,Yinchuan 750001,China)
机构地区:[1]宁夏医科大学总医院中医科,宁夏银川750004 [2]宁夏银川市中医医院,宁夏银川750001
出 处:《宁夏医学杂志》2019年第12期1057-1060,共4页Ningxia Medical Journal
基 金:宁夏自然科学基金资助项目(2018AAC03166);宁夏医科大学校级课题(XM201440)
摘 要:目的使用动物实验研究探索健脾补肾解毒方通过下调lewis肺癌小鼠脾脏中的CD4^+CD25^+调节性T细胞的比例来达到抑制肿瘤的作用机制。方法将40只C57BL/6J小鼠,随机分为正常组、荷瘤模型组、化疗组(顺铂组)、中药组(健脾补肾解毒方)、联合组(健脾补肾解毒方+顺铂组),给药14 d后处死小鼠,剥离脾脏,用流式细胞仪检测小鼠脾脏CD4^+CD25^+的含量。结果小鼠脾脏CD4^+CD25^+调节T细胞含量与正常组比较,中药组、荷瘤模型组、化疗组比率明显升高,差异有统计学意义(P<0.05);联合组比率虽高于正常组,但差异无统计学意义(P>0.05);与荷瘤模型组比较,化疗组、中药组、联合组比率明显降低,差异有统计学意义(P<0.05)。结论健脾补肾解毒方通过下调lewis肺癌小鼠脾脏中CD4^+CD25^+调节性T细胞的比例能达到抑制肿瘤的作用。Objective To explore the mechanism of Jianpi Bushen Jiedu recipe in inhibiting tumors by reducing the proportion of CD4^+CD25^+ regulatory T cells(Treg) in the spleen of Lewis lung cancer mice through animal experiments.Methods A total of 40 C57 BL/6 J mice were randomly divided into normal group,model group,chemotherapy group(cisplatin group),traditional Chinese medicine group(Jianpi Bushen Jiedu recipe),combined group(Jianpi Bushen Jiedu recipe + cisplatin group). After 14 days,the mice were executed and their spleens were stripped. The content of CD4^+CD25^+ in mouse spleens were detected by flow cytometry.Results Compared with the normal group,the ratio of CD4^+CD25^+ regulation T cells in traditional Chinese medicine group,model group,chemotherapy group was significantly higher(P<0.05),although the ratio in combined group was higher than that in the normal group,but the difference was not statistically significant(P>0.05). Compared with the model group,the chemotherapy group,the tradition Chinese medicine group,the combined group was significantly lower,the difference was statistically significant(P<0.05).Conclusion Jianpi Bushen Jiedu recipe can inhibit the tumor by decreasing the proportion of CD4^+CD25^+regulatory T cells in the spleen of Lewis lung cancer mice.
关 键 词:LEWIS肺癌 小鼠 健脾补肾解毒方 CD4^+CD25^+调节 T细胞
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