梅毒孕妇血清和体液梅毒螺旋体DNA检测分析  

Detection of Treponema pallidum DNA in serum and body fluid of pregnant women with syphilis

作　　者：陈雪 曹文延[2] 张玉蓉[3] CHEN Xue;CAO Wen-yan;ZHANG Yurong(The Traditional Chinese Medicine of Ningxia,Yinchuan 750021,China;The Guyuan Peoples Hospital of Yinchuan,Guyuan 756000,China;The Medicine Experiment Center of Gereral Hospital of Ningxia Medicine University,Yinchuan750004,China)

机构地区：[1]宁夏回族自治区中医医院检验科,宁夏银川750021 [2]宁夏固原市人民医院检验科,宁夏固原756000 [3]宁夏医科大学总医院医学检验中心,宁夏银川750004

出　　处：《宁夏医学杂志》2019年第12期1064-1066,共3页Ningxia Medical Journal

摘　　要：目的探究梅毒孕妇治疗后血清、唾液和乳汁标本中梅毒螺旋体DNA(TP-DNA)水平,对孕妇体液的传染性进行评价,为产后能否哺乳提供理论依据。方法选取梅毒孕妇78例,均实施3个规范化疗程治疗。根据疗效分为有效组36例和无效组42例。采用荧光定量PCR技术对2组梅毒孕妇的血清、唾液和乳汁标本中的TP-DNA进行检测,同时实施梅毒甲苯胺红不加热血清试验(TRUST)检测滴度。结果 78例梅毒孕妇在治疗3个疗程后,血清、唾液和乳汁标本中的TP-DNA阳性率分别为14.1%、11.5%和12.8%,无效组血清、唾液和乳汁标本中的TP-DNA阳性率均高于有效组(P<0.05),2组组内血清、唾液和乳汁标本中的TP-DNA阳性率比较差异均无统计学意义(P>0.05)。不同血清TRUST滴度患者间血清、唾液和乳汁标本中的TP-DNA阳性率差异均有统计学意义(P<0.05),且随着滴度升高,各种标本的TP-DNA阳性率随之升高。结论梅毒孕妇在接受规范治疗后的体液传染性与其TP-DNA载量相关,通过统计学分析提示,唾液和乳汁可代替血清进行TP-DNA检测,不仅方便取材,孕妇更易接受。各项体液TP-DNA均为阴性者可考虑实施母乳喂养。Objective To explore the level of Treponema pallidum DNA(TP-DNA) in serum,saliva and milk samples of pregnant women with syphilis after treatment,and to evaluate the infectivity of pregnant women’s body fluids,so as to provide theoretical basis for postpartum lactation.Methods 78 pregnant women with syphilis were selected and received three standardized treatments. According to the curative effect,the patients were divided into the effective group(n=36) and the ineffective group(n=42). TP-DNA in serum,saliva and milk samples of pregnant women with syphilis were detected by fluorescent quantitative PCR. TRUST test was also carried out to detect the titer.Results After three standardized courses of treatment,the positive rates of TP-DNA in serum,saliva and milk samples were 14.1%,11.5% and 12.8%,respectively. The positive rates of TP-DNA in serum,saliva and milk samples of ineffective group were higher than those of effective group(P<0.05),but there was no significant difference between the two groups in the positive rates of TP-DNA in serum,saliva and milk samples(P>0.05). The positive rates of TP-DNA in serum,saliva and milk samples were significantly different among patients with different serum TRUST titers(P<0.05). With the increase of TRUST titers,the positive rates of TP-DNA in various samples increased.Conclusion The humoral infectivity of syphilis pregnant women after receiving standard treatment is related to their TP-DNA load. Statistical analysis shows that saliva and milk can replace serum for TP-DNA detection,which is not only convenient for samples,but also easier for pregnant women to accept. Breastfeeding can be carried out in patients with negative TP-DNA in all body fluids.

关 键 词：梅毒 孕产妇 体液 梅毒螺旋体DNA 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]