佳蓉片中6种乌头类生物碱的定量研究  

Quantitative study on 6 aconitoid alkaloids in Jiarong Tablets

作　　者：王媛 袁娟丽 何悦 刘岩 WANG Yuan;YUAN Juanli;HE Yue;LIU Yan(Department of Pharmacy,983 Hospital of the Joint Logistics Support Force of the Chinese People′s Liberation Army,Tianjin 300142,China;Department of Pharmacy,the Second Affiliated Hospital of Air Force Military Medical University,Xi′an 710038,China;Department of Chinese Materia Medica and Natural Medicines,Air Force Medical University,Xi′an 710032,China)

机构地区：[1]中国人民解放军联勤保障部队第九八三医院药剂科,天津300142 [2]空军军医大学第二附属医院药剂科,西安710038 [3]空军军医大学中药和天然药物学教研室,西安710032

出　　处：《西北药学杂志》2020年第1期38-41,共4页Northwest Pharmaceutical Journal

基　　金：陕西省重点研发计划项目(编号:2017SF-299)

摘　　要：目的分析佳蓉片中苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、乌头碱和次乌头碱6种乌头类生物碱的含量。方法采用HPLC法。色谱条件:Kromasil C 18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-四氢呋喃(25∶15)以及0.1 mol·L^-1醋酸铵溶液(每1000 mL加冰醋酸0.5 mL),梯度洗脱;流速为0.8 mL·min^-1;柱温为25℃;检测波长为235 nm。结果苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、乌头碱和次乌头碱分别在4.32~432.40,1.29~128.83,1.08~108.46,1.77~177.30,0.78~78.40和1.19~119.04μg·mL^-1范围内与峰面积线性关系良好;平均回收率分别为96.46%,98.26%,98.03%,98.23%,98.86%和98.70%;RSD值分别为1.50%,1.20%,1.27%,1.06%,1.50%和1.19%;10批制剂中苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、乌头碱和次乌头碱的含量范围分别为100.32~119.18,9.35~16.02,7.99~10.87,1.54~1.85,0.87~0.99和2.21~3.27μg·g^-1。结论该方法简单、准确、灵敏、重复性好,可作为佳蓉片中乌头类生物碱成分的定量方法。Objective To analyze the contents of 6 kinds of aconitoid alkaloids including benzoylmesaconine,benzoylaconitine,benzoylhypacoitine,mesaconitine,aconitine and hypaconitine in Jiarong Tablets.Methods HPLC was adopted,and the chromatographic column was Kromasil C 18(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-tetrahydrofuran(25∶15)and 0.1 mol·L^-1 ammonium acetate solution(0.5 mL glacial acetic acid per 1000 mL)in a gradient mode.The flow rate was 0.8 mL·min-1.The column temperature was 25℃ and the detection wavelength was 235 nm.Results The mass of benzoylmesaconine,benzoylaconitine,benzoylhypacoitine,mesaconitine,aconitine and hypaconitine showed good linear relationships with peak area in the range of 4.32-432.40,1.29-128.83,1.08-108.46,1.77-177.30,0.78-78.40 and 1.19-119.04μg·mL^-1,respectively.Their average recoveries were 96.46%,98.26%,98.03%,98.23%,98.86%and 98.70%,respectively with RSD were 1.50%,1.20%,1.27%,1.06%,1.50%and 1.19%,respectively.The content range of benzoylmesaconine,benzoylaconitine,benzoylhypacoitine,mesaconitine,aconitine and hypaconitine in 10 batches of preparation were 100.32-119.18,9.35-16.02,7.99-10.87,1.54-1.85,0.87-0.99 and 2.21-3.27μg·g^-1,respectively.Conclusion This method is simple,accurate,sensitive and reproducible.And it can be used as a quantitative method for aconitoid alkaloids in Jiarong Tablets.

关 键 词：佳蓉片 生物碱 新乌头碱 乌头碱 次乌头碱 HPLC法 

分 类 号：R927.2[医药卫生—药学]