碳纤维微电极负载金纳米粒子用于芦荟多糖的在体动态监测  

In vivo Dynamic Detection of Aloe Polysaccharides Using Carbon Fiber Microelectrodes Modified with Gold Nanoparticles

作　　者：王婷婷[1] 李元[1] 杨莉莉 鲍昌昊 程寒[1,2] WANG Tingting;LI Yuan;YANG Lili;BAO Changhao;CHENG Han(School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan 430074,China;National Demonstration Center for Experimental Ethnopharmacology Education,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074 [2]中南民族大学民族药学国家级实验教学示范中心,武汉430074

出　　处：《高等学校化学学报》2020年第1期87-93,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21205144);中央高校基本科研业务费专项资金项目(批准号:CZY18036);民族医药学会项目(批准号:2017KYXM-M106-81);中南民族大学研究生学术创新基金后期资助项目资助~~

摘　　要：建立了一种对活体芦荟储水凝胶组织中芦荟多糖进行在体实时动态检测的电化学方法.通过在活体芦荟植株凝胶中嵌入高灵敏度的纳米金粒子修饰的碳纤维微电极,对不同光照条件下芦荟植株中的芦荟多糖进行在体动态监测,进而评价植株应激外界环境下芦荟多糖含量的实时变化.实验结果表明,纳米金粒子修饰的碳纤维微电极对芦荟储水凝胶组织中芦荟多糖具有显著的电催化活性,不同光照环境下生长的芦荟植株中芦荟多糖含量的动态变化具有显著的统计学差异.该修饰电极可用于芦荟植株中芦荟多糖动态变化的在体实时监测,使电化学检测方法有望成为监测植物光合作用程度的一种快捷检测手段.An electrochemical method for real-time dynamic detection of aloe polysaccharide in water storage tissue gel layer of aloe leaves was established.The aloe polysaccharides in aloe plants under different growth conditions were monitored in vivo by high-sensitivity carbon fiber microelectrodes modified with gold nanoparticles(AuNPs/CFME),to evaluate the real-time changes of aloe polysaccharides in the environment under stress.The experimental results show that the AuNPs/CFME has significant electrocatalytic activity to aloe polysaccharides in aloe water storage tissue.The dynamic changes of aloe polysaccharide in aloe plants under different growth environments have significant statistical differences.The modified electrode was success-fully applied to the dynamic change of aloe polysaccharide in aloe plants for real-time monitoring,which made the electrochemical detection method a promising method for monitoring the photosynthesis intensity of plants.

关 键 词：碳纤维微电极 纳米金粒子 芦荟多糖 动态监测 

分 类 号：O657.1[理学—分析化学]