紫红笛鲷血细胞发生研究  

Characterization of Hematopoiesis in Red Snapper Lutjanus argentimaculatus

作　　者：钟爱华 牟毅 高航 王文鸿 ZHONG Aihua;MOU Yi;GAO Hang;WANG Wenhong(School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学水产学院

出　　处：《水产科学》2020年第1期48-54,共7页Fisheries Science

基　　金：国家自然科学基金资助项目(31700322);浙江省“水产”一流学科开放课题项目(20160012)

摘　　要：将体质量510~620 g的紫红笛鲷用间氨基苯甲酸乙酯甲磺酸盐麻醉后解剖,取出头肾、体肾、肝脏、脾脏,用干净的刀片居中横切、纵切,印片和涂片用Wright-Giemsa染液染色10 min,观察头肾、体肾、脾脏、肝脏4个组织印片以及外周血涂片。试验结果显示,头肾、体肾、脾脏是其造血器官。头肾能发育生成各类型血细胞,体肾能生成红细胞、淋巴细胞、粒细胞和单核细胞,脾脏能生成淋巴细胞。肝脏中未发现幼稚型血细胞。红细胞发育经过原红细胞、早幼红细胞、中幼红细胞、晚幼红细胞和红细胞5个阶段;原粒细胞发育至早幼粒细胞后,分化为嗜碱性中幼粒细胞、嗜酸性中幼粒细胞和中性中幼粒细胞,最后发育成嗜碱性粒细胞、嗜酸性粒细胞和中性粒细胞;淋巴细胞和单核细胞发育经过原始、幼稚和成熟3个阶段。血细胞在发育过程中,胞体逐渐变小,细胞核逐渐变小,染色质由疏松到致密;粒细胞中,颗粒由少到多。Red snapper Lutjanus argentimaculatus weighing 510-620 g was anaesthetized with MS-222 and dissected to sample head kidney,pronephros,liver and spleen which were dissected with a clean blade.The prints and smears were stained with Wright-Giemsa staining solution for 10 minutes,and the head kidney,pronephros,spleen,liver and peripheral blood were observed.Head kidney,pronephros and spleen are their hematopoietic organs,and head kidney can develop into various types of blood cells,body kidney can produce red blood cells,lymphocytes,granulocytes and monocytes,and spleen can produce lymphocytes.The developments of erythrocyte,granulocyte,lymphocyte,and monocyte were found in the pronephros,and the developments of erythrocyte,granulocyte,lymphocyte and monocyte were observed in the kidney and the developments of lymphocyte in the spleen.There were no immature blood cells in liver smears,no doubt that the liver was not the hematopoietic organ.The developmental stage of erythroid was subdivided into five stage:proerythroblast,basophilic erythroblast,polychromatic erythroblast,orthochromatic erythroblast and erythrocyte.The primary granulocytes developed into early granulocytes and then differentiated into basophil,eosinophil and neutrophil.The developmental stage of lymphocyte and monocyte was subdivided:primitive,young and mature.During the development,the cell body and nucleus were smaller gradually in size,and the chromatin was changed from loose to dense.In granulocytes,particles were changed from less to more.

关 键 词：紫红笛鲷 血细胞 血细胞发生 细胞化学染色 

分 类 号：S917.4[农业科学—水产科学]