30种中草药对鲟鱼源海豚链球菌的体外抑菌作用  

Antibacterial Effects of 30 Kinds of Chinese Herbal Medicines Against Pathogen Streptococcus iniae from Sturgeon Hybrid Huso dauricus♀× Acipenser schrenckii♂ in Vitro

作　　者：姚丽 董春燕[1,2] 陈江凤 黎明[3] 邵俭 安苗[1,2] 王常安 王静波[5] 程振涛 姜海波[1,2,6] 文明 YAO Li;DONG Chunyan;CHEN Jiangfeng;LI Ming;SHAO Jian;AN Miao;WANG Chang′an;WANG Jingbo;CHENG Zhentao;JIANG Haibo;WEN Ming(College of Animal Sciences,Guizhou University,Guiyang 550025,China;Breeding and Reproduction in the Plateau Mountainous Region(Guizhou University),Ministry of Education,Guiyang 550025,China;School of Marine Sciences,Ningbo University,Ningbo 315211,China;Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;Beijing Fisheries Technical Extension Station,Beijing 100176,China;Key Laboratory for Animal Diseases and Veterinary Public Health of Guizhou Province,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 [3]宁波大学海洋学院,浙江宁波315211 [4]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [5]北京市水产技术推广站,北京100176 [6]贵州省动物疫病与兽医公共卫生重点实验室,贵州贵阳550025

出　　处：《水产科学》2020年第1期111-116,共6页Fisheries Science

基　　金：贵州省科技支撑计划项目(黔科合支撑[2016]2502号,[2016]2511号,[2017]2532号);贵州省科技平台及人才团队计划项目(黔科合平台人才(2018)5253号);贵州省科技创新人才团队项目(黔科合人才团队(2015)4016号);北京市鲟鱼、鲑鳟鱼创新团队项目(BAIC08-2018)

摘　　要：为研究黄连等30种中草药对鲟鱼源海豚链球菌的抑菌、杀菌作用,通过水煎煮法对30种中草药进行提取,采用牛津杯打孔法,在观察鲟鱼源海豚链球菌对30种中草药敏感性的基础上,进一步采用二倍稀释法测定中草药对鲟鱼源海豚链球菌的最小抑菌质量浓度和最小杀菌质量浓度。试验结果表明,鲟鱼源海豚链球菌对黄连、黄芩、连翘、丁香高度敏感,其中黄连的体外抑菌效果最佳,抑菌圈直径为(28.35±0.65) mm;对秦皮、山楂、女贞子、苦楝皮、石榴皮等均中度敏感;对丹参、猪苓、苍术、茯苓、贯众和生首乌低度敏感。黄连、黄芩、连翘、丁香、秦皮对鲟鱼源海豚链球菌的最小抑菌质量浓度和最小杀菌质量浓度分别为1.95 mg/mL和3.90 mg/mL、1.95 mg/mL和15.62 mg/mL、3.90 mg/mL和7.81 mg/mL、3.90 mg/mL和15.62 mg/mL、7.81 mg/mL和7.81 mg/mL,其余中草药对病原菌的最小抑菌质量浓度和最小杀菌质量浓度均高于15.62 mg/mL和31.25 mg/mL。综上可知,黄连、黄芩、连翘、丁香、秦皮对鲟鱼源海豚链球菌具有较好的防治效果。The antibacterial effects of 30 kinds of Chinese herbal medicines including Coptis chinensis, Scutellaria baicalensis, Forsythia suspensa, and the others on pathogen Streptococcus iniae from sturgeon hybrid Huso dauricus ♀× Acipenser schrenckii ♂ were investigated in vitro by water decoction and punched by Oxford cup, and the minimal inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of 30 kinds Chinese herbs were measured by microdilution procedure. The results showed that the best inhibitory effects with MIC of(28.35±0.65) mm were observed in C. chinensis, and 5 kinds of Chinese herbs inhibited the growth of S. iniae,with MIC of 1.95 mg/mL and MBC of 3.90 mg/mL in C. chinensis, MIC of 1.95 mg/mL and MBC of 15.62 mg/mL in S. baicalensis, MIC of 3.90 mg/mL and MBC of 7.81 mg/mL in F. suspense, MIC of 3.90 mg/mL and MBC of 15.62 mg/mL in Syzygium aromaticum, and MIC of 7.81 mg/mL and MBC of 7.81 mg/mL in Fraxinus rhynchophylla. The MIC and the MBC of the other Chinese herbal medicines against the pathogenic bacterium were all higher than 15.62 mg/mL and 31.25 mg/mL. In conclusion, C. chinensis, S. baicalensis, F. suspense, S. aromaticum, and F. rhynchophylla showed effectively antimicrobial alternatives to control the S. iniae from the sturgeon hybrid H. dauricus ♀× A. schrenckii ♂.

关 键 词：杂交鲟 中草药 海豚链球菌 抑菌效果 

分 类 号：S948[农业科学—水产养殖]