海南无乳链球菌的毒力基因与耐药特性分析  

Virulence Genes and Drug Resistance of Pathogen Streptococcus agalactiae in Hainan

作　　者：王爱媛 郑立新 蒲文渊 杨诺 曾纪锋[1] 郭桂英 李迁 郑继平[1] WANG Aiyuan;ZHENG Lixin;PU Wenyuan;YANG Nuo;ZENG Jifeng;GUO Guiying;LI Qian;ZHENG Jiping(Institute of Life Sciences and Pharmacy,Hainan University,Haikou 570228,China;Hospital of Hainan University,Haikou 570228,China;Academic Affairs Office of Hainan University,Haikou 570228,China;Network and Educational Technology Center of Hainan University,Haikou 570228,China)

机构地区：[1]海南大学生命科学与药学学院,海南海口570228 [2]海南大学校医院,海南海口570228 [3]海南大学教务处,海南海口570228 [4]海南大学网络与教育技术中心,海南海口570228

出　　处：《水产科学》2020年第1期117-123,共7页Fisheries Science

基　　金：国家自然科学基金资助项目(31460699);海南自然科学基金创新研究团队项目(2017CXTD005);海南自然科学基金面上项目(317071)

摘　　要：无乳链球菌可感染人与其他动物,是一种重要的致病菌。为探明海南地区无乳链球菌的毒力基因携带率及耐药情况,以2017年间实验室所采集鉴定的18株无乳链球菌(人源4株,鱼源14株)为样本,利用PCR技术和抗菌药物药敏纸片进行毒力基因、耐药基因的扩增和药物敏感性的检测,结果发现,常见毒力基因阳性检出率较高,其中有11种毒力基因均100%携带,另外3种基因阳性率也超过66.7%;耐药基因扩增结果显示,除四环素类和氨基糖苷类耐药基因均呈阴性以外,其余耐药基因阳性检出率较高(44.4%~100%);而药敏检测显示,无乳链球菌对β-内酰胺类、大环内酯类、林可胺类抗生素耐药性高,而对于氨基糖苷类、喹诺酮类、四环素类抗生素敏感性高。总体上,药敏情况和耐药基因的检测结果一致。研究结果发现,分离得到的不同来源的无乳链球菌中毒力基因检出频率高,耐药性强,需要引起高度重视,试验结果将为无乳链球菌的防治提供重要的参考。Virulence genes and resistance genes were screened in 18 strains of Streptococcus agalactiae as an important zoonotic and human pathogen collected from women and tilapia in 2017 via 14 virulence genes by PCR,and sequencing and amplification and drug sensitivity in Hainan was determined by bacterial drug susceptibility paper in order to investigate its virulence genes,carrying rate and drug resistance of the pathogen.The results showed that there was high positive detection rate in the virulence genes among the tested strains,detection of 11 virulence genes from all samples,with as high as 66.7%of positive rate in the remaining genes.The amplification of drug resistance genes revealed that the positive rate of the rest genes was high(44.4%-100%)except for the negative resistance genes against tetracycline and amino acid glycoside.S.agalactiae was shown to have high tolerance toβ-lactams,macrolides,and lincosamides and high sensitivity to the amino acid glycoside,quinolone,and tetracycline,indicating that the drug sensitivity is consistent with the detection of drug resistance genes.Therefore,it should be concerned about and judged the high frequency of virulence genes and the high drug resistance among S.agalactiae isolated in Hainan in traditional antibiotic treatment.

关 键 词：无乳链球菌 毒力基因 耐药基因 药敏检测 

分 类 号：S948[农业科学—水产养殖]