猪SPATA6基因的多态性与精液品质性状的关联分析  

Association analysis of SPATA6 gene polymorphism and semenquality in porcine

作　　者：周佳伟 吴俊静[1] 乔木[1] 刘贵生[1] 彭先文[1] 梅书棋[1] ZHOU Jia-wei;WU Jun-jing;QIAO Mu;LIU Gui-sheng;PENG Xian-wen;MEI Shu-qi(Hubei Key Laboratory of Animal Embryo and Molecular Breeding/Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室

出　　处：《湖北农业科学》2019年第24期163-166,169,共5页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心创新团队项目（2019-620-000-001-18）;国家生猪产业技术体系项目（CARS-35）;湖北省农业科技创新行动项目（2018skjcx05）;湖北省自然科学基金创新群体项目（2018CFA014）;湖北省技术创新专项重大项目（2019ABA084）;湖北省农业科学院青年科学基金项目（2018NKYJJ09）

摘　　要：公猪精液品质直接影响到猪场经济效益,通过转录组测序发现SPATA6基因在猪性成熟前后的睾丸组织中差异表达,并发现其内含子区域内存在多个SNP位点。通过SNaPshot的方法分别对杜洛克猪和大白猪中SPATA6基因的rs331255092和rs341061477位点进行分型,并与精液品质性状进行关联分析。结果表明,在杜洛克猪和大白猪中rs331255092和rs341061477位点均与精子畸形率显著相关,此外,在大白猪中rs331255092和rs341061477位点也与精液量显著相关。猪SPATA6基因的rs331255092和rs341061477位点对猪的精液品质存在潜在调控作用。The quality of boar semen directly affects the economic benefits of pig farms. Transcriptome sequencing showed that SPATA6 gene was differentially expressed in testicular tissues of porcine sexually mature and immature, and multiple SNP sites were found in its intron region. In this study, rs331255092 and rs341061477 loci of SPATA6 gene were identified by SNaPshot method in Duroc pig and Large White pig. The results showed that rs331255092 and rs341061477 were significantly correlated with sperm abnormality rate in Duroc pig and Large White pig. In addition, rs331255092 and rs341061477 were also significantly correlated with semen volume in Large White pig. In summary, rs331255092 and rs341061477 have potential regulatory effects on semen quality in pigs.

关 键 词：猪 SPATA6基因 SNP 精子畸形率 精液量 

分 类 号：S828[农业科学—畜牧学]