朱顶红褪绿环斑病毒在细角带蓟马中的增殖规律研究  

Proliferative Rule of Hippeastrum chlorotic ringspotvirus in Taeniothrips eucharii

作　　者：吴家琦 徐烨[2] 李永忠[3] 刘雅婷[1] WU Jiaqi;XU Ye;LI Yongzhong;LIU Yating(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学植物保护学院,云南昆明650201 [3]云南农业大学烟草学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2020年第1期15-19,61,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31471828,31260451);云南省科技厅重点项目(2018FA019)

摘　　要：【目的】朱顶红褪绿环斑病毒(Hippeastrum chlorotic ringspot virus,HCRV)危害严重,细角带蓟马(Taeniothrips eucharii)是其主要的传播媒介。明确HCRV病毒粒子在细角带蓟马体内的增值规律,为HCRV防控提供理论依据。【方法】应用实时荧光定量PCR技术(qRT-PCR),结合HCRV N基因和细角带蓟马βactin基因特异性引物,选用SYBR Green方法对获毒后不同虫龄细角带蓟马体内的HCRV含量进行定量分析。【结果】所建立的实时荧光定量方法可以准确特异地对细角带蓟马中的HCRV进行相对定量,发现1龄若虫的HCRV核酸含量最低,1龄若虫、2龄若虫及蛹中的病毒含量没有统计学差异,成虫的HCRV核酸含量最高。【结论】细角带蓟马在获毒后,体内HCRV核酸含量随着细角带蓟马的发育而增加。[Purpose]Hippeastrum chlorotic ringspot virus(HCRV)can cause serious damage and is transmited by Taeniothrips eucharii.The aim of this study was to provide the theoretical evidence for controlling HCRV.[Method]In this study,SYBR Green method of real-time fluorescent quantitative PCR(qRT-PCR)was used,combined with the specific primers for HCRV N gene andβactin gene of T.eucharii for quantitative analysis of HCRV content in different treatments.[Reault]This method could accurately and specifically quantify the HCRV virus in T.eucharii.quantitative of HCRV RNA.The concentrate of HCRV was the lowest in 1st larvae.There was no statistical difference in 1^st larva,2nd larva and pura.And the concentrate of HCRV was the highest in adults.HCRV was growing gradually after the 1^st larvae acquire HCRV.[Conclusion]This indicated that T.eucharii transmitted HCRV as a persistent-propagative manner.

关 键 词：正番茄斑萎病毒属 朱顶红褪绿环斑病毒 细角带蓟马 荧光定量PCR 

分 类 号：S433.89[农业科学—农业昆虫与害虫防治]