榛子花的质量标准研究  

Research on Quality Standards of Corylus heterophylla Fisch

作　　者：孙艳杰[1] 王路宏[1] 王皓[1] 赵磊[1] SUN Yan-jie;WANG Lu-hong;WAGN Hao;ZHAO Lei(Siping Institute for Food and Drug Control,Jilin Siping 136000,China)

机构地区：[1]四平市食品药品检验所

出　　处：《中国药物评价》2019年第6期438-441,共4页Chinese Journal of Drug Evaluation

基　　金：吉林省地方药材标准化2017项目(榛子花);吉林省地方药材标准化2018项目(榛子花饮片)

摘　　要：目的:建立榛子花的薄层鉴别与榛子花中槲皮素、山柰素含量测定的高效液相色谱方法。方法:采用薄层色谱用硅胶G板对榛子花中槲皮素进行定性鉴别;采用色谱条件:色谱柱为Sepax BR-C18(4. 6 mm×250 mm,5μm);流动相为甲醇-0. 4%磷酸(48∶52);检测波长为360 nm;柱温:30℃;流速为1. 0 m L·min^-1。结果:采用的薄层色谱鉴别方法重现性好,专属性强。含量测定槲皮素、山柰素的线性良好(r> 0. 999 5);回收率分别为98. 31%(RSD 1. 3%)和96. 80%(RSD 0. 96%)。结论:建立质量标准方法简单、重现性好,为榛子花的质量控制提供测定依据。Objective: To establish a thin layer chromatography identification method for hazelnut flowers and to establish a HPLC method for the determination of quercetin and kaempferol in Corylus heterophylla Fisch. Methods: The quercetin in Corylus heterophylla Fisch was qualitatively identified on silica gel G plate of thin-layer chromatography. Chromatographic conditions were used: Inertsil ODSSP C18( 4. 6 mm × 250 mm,5 μm);mobile phase was methanol-0. 4% phosphoric acid( 48∶ 52) The detection wavelength was 360 nm;the column temperature was 30 ℃;and the flow rate was 1. 0 m L·min^-1. Results: The TLC identification method has good reproducibility and strong specificity. The linearity of quercetin and kaempferol in the content determination was good( r > 0. 999 5). The recoveries were 98. 31%( RSD 1. 3%) and 96. 80%( RSD 0. 96%),respectively. Conclusion: The establishment method is simple,prepared and reproducible,and can provide a reference for the application of the medicine.

关 键 词：榛子花 薄层色谱 高效液相色谱 槲皮素 山柰素 含量测定 

分 类 号：R284.1[医药卫生—中药学] R926[医药卫生—中医学]