芎麻汤提取物对肝阳上亢兼瘀血型偏头痛模型大鼠脑组织中5-HT、CGRP的影响  

Effects of the Extract of Xiongmatang on 5-HT and CGRP in Brain Tissue of Rats with Liver-yang Hyperactivity and Blood Stagnation Migraine

作　　者：刘顶鼎 杨菁 刘冬 郭建生 毛效贤[1] 曾贵荣 周英 LIU Dingding;YANG Jing;LIU Dong;GUO Jiansheng;MAO Xiaoxian;ZENG Guirong;ZHOU Ying(School of Pharmacy,Guizhou University of TCM,Guiyang 550025,China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Beijing 100050,China;School of Pharmacy,Hunan University of TCM,Changsha 410208,China;Drug Safety Evaluation Research Center of Hunan Province/Key Laboratory of New Drug Pharmacodynamics and Safety Evaluation of Hunan Province,Changsha 410331,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]天然药物活性物质与功能国家重点实验室,北京100050 [3]湖南中医药大学药学院,长沙410208 [4]湖南省药物安全评价研究中心/新药药效与安全性评价湖南省重点实验室,长沙410331

出　　处：《中国药房》2020年第1期24-29,共6页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81860844);贵州省科技计划项目(No.黔科合基础〔2016〕1016、No.黔科合基础〔2019〕1038号);贵州省教育厅青年科技人才成长项目(No.黔教合KY字〔2016〕185);贵州省中医药管理局中医药、民族医药科学技术研究课题(No.QZYY-2016-001)

摘　　要：目的:研究芎麻汤提取物对肝阳上亢兼瘀血型偏头痛模型大鼠脑组织中5-羟色胺(5-HT)、降钙素基因相关肽(CGRP)的影响。方法:将50只雄性自发性高血压(SHR)大鼠按血压随机分为模型对照组、正天丸阳性对照组(1.6 g/kg)和芎麻汤提取物低、中、高剂量组(4.5、9.0、18.0 g/kg,以生药量计),每组10只;并另取10只正常大鼠作为正常对照组。给药组大鼠分别灌胃相应药物,每天1次,连续28 d;正常对照组和模型对照组大鼠灌胃等体积生理盐水。末次给药后,除正常对照组外,其余各组大鼠均采用电刺激三叉神经节(10 min)法复制肝阳上亢兼瘀血型偏头痛大鼠模型,并于造模后再维持给药1次。30 min后,观察大鼠一般行为学及舌质情况,测定大鼠血压,采用酶联免疫吸附法测定大鼠脑组织中5-HT、CGRP含量,并采用Western blot法测定大鼠脑组织中CGRP蛋白表达水平。结果:与正常对照组比较,模型对照组大鼠出现眼结膜颜色加深变红、过度理毛、甩头等行为学变化,大部分大鼠舌质紫暗,并且其收缩压和脑组织中CGRP含量及蛋白表达水平均明显升高(P<0.05或P<0.01),脑组织中5-HT含量显著降低(P<0.01)。与模型对照组比较,各给药组大鼠的上述行为学变化及舌质变化均明显改善;正天丸阳性对照组和芎麻汤提取物高剂量组大鼠收缩压、脑组织中CGRP含量及蛋白表达水平显著降低(P<0.05);正天丸阳性对照组和芎麻汤提取物中、高剂量组大鼠脑组织中5-HT含量显著升高(P<0.05)。结论:芎麻汤提取物对肝阳上亢兼瘀血型偏头痛模型大鼠有明显的保护作用,其机制可能与降低脑组织中CGRP含量、升高脑组织中5-HT含量有关。OBJECTIVE:To study the effects of the extract of Xiongmatang on 5-HT and CGRP in brain tissue of rats with liver-yang hyperactivity and blood stagnation migraine. METHODS:Fifty male spontaneously hypertension(SHR) rats were randomly divided into model control group,Zhengtian pill positive control group(1.6 g/kg),the extract of Xiongmatang lowdose,medium-dose and high-dose groups(4.5,9.0,18.0 g/kg,by crude drug),with 10 rats in each group. Another 10 normal rats were taken as normal control group. Administration group was given relevant medicine intragastrically,once a day,for consecutive 28 d. Normal control group and model control group were given equal volume normal saline intragastrically. After last administration,except for normal control group,other groups were used to stimulate trigeminal ganglia(10 min)to establish liveryang hyperactivity and blood stagnation migraine model,and maintained the administration once more after making the model. 30 min later,general behavior and tongue quality of rats were observed,and the blood pressure was measured;the contents of 5-HT and CGRP in cerebral tissue of rats were determined by ELISA. The protein expression of CGRP in cerebral tissue of rats were determined by Western blot method. RESULTS:Compared with the normal control group, the rats in the model control group had behavioral symptoms,such as the color of conjunctiva deepened and reddened, excessive hairdressing,head flicking,and most of the rats had purple tongue;the systolic pressure and the content and protein expression of CGRP were all increased obviously(P<0.05 or P<0.01),while the content of 5-HT in cerebral tissue was decreased obviously(P<0.01). Compared with model control group,general behavior and tongue quality of rats were improved significantly in administration groups. Systolic pressure,the content and the protein expression of CGRP in cerebral tissue of rats were decreased significantly in Zhengtian pill positive control group and the extract of Xiongmatang high-dose group(P<0.05), while the con

关 键 词：芎麻汤提取物 肝阳上亢兼瘀血型偏头痛模型 自发性高血压大鼠 5-羟色胺 降钙素基因相关肽 

分 类 号：R285[医药卫生—中药学]