果树芽再生研究进展  

Advances in researches on bud regeneration in fruit crops

作　　者：刘警 刘平[1,2] 刘孟军 李东东[3] LIU Jing;LIU Ping;LIU Mengjun;LI Dongdong(Research Center of Chinese Jujube,Hebei Agricultural University,Baoding 071001,Hebei,China;Institute of Jujube Industry Technology of Hebei,Baoding 071001,Hebei,China;College of Horticulture,Hebei Agricultural University,Baoding 071001,Hebei,China)

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001 [2]河北省枣产业技术研究院,河北保定071001 [3]河北农业大学园艺学院,河北保定071001

出　　处：《果树学报》2020年第1期115-129,共15页Journal of Fruit Science

基　　金：国家科技支撑计划项目(2013BAD14B0301);国家自然科学基金(31372029);河北省自然科学基金(C2014204047);河北省科技支撑计划(16226313D-1);河北省科技条件建设项目(16967645D);农业科研杰出人才培养计划(2016-2020)

摘　　要：芽再生在植物组培快繁、遗传转化及诱变育种等研究中应用广泛。笔者在本课题组20多年从事枣组培和芽再生研究的基础上,综述了影响果树芽再生的主要因素,包括基因型、外植体类型、生长调节剂种类及配比、光照条件、基本培养基类型、氮源、碳源、AgNO_3和干化处理等,提出了果树芽再生存在的主要问题和对策措施、前景展望,以期为提高果树芽再生效率提供参考。Bud regeneration is widely used in plant tissue culture,genetic transformation and mutation breeding.On the basis of more than 20 years’research on tissue culture and bud regeneration of Chinese jujube,the main factors affecting bud regeneration of fruit trees were summarized in this paper,including genotype,explant types,types and proportions of growth regulators,light conditions,basic medium types,nitrogen and carbon sources,AgNO3 and drying treatment,etc.And also,the main problems and solutions as well as prospect in the study on bud regeneration of fruit crops were put forward in order to provide references for improving the bud regeneration efficiency of horticultural plants.Among them,genotype is the most important factor affecting bud regeneration.Cells are omnipotent,and after dedifferentiation and redifferentiation,we can obtain regenerated buds that can continue to grow and develop entire plants.However,not all genotypes can show the omnipotence of cells.The response of different genotypes to bud induction is also different,and the efficiency of bud induction varies greatly with genotypes.This may be due to the difference in bud regeneration ability between different genotypes or the different sensitivity between different genotypes to external stimuli,and the different optimal stimulus conditions,resulting in the difference of bud regeneration efficacy.The type and development degree of explants are one of the key factors affecting bud regeneration ability to produce the same genotype plants.Different explant types and different parts of explants result in different bud regeneration ability.The regeneration ability of tissues and parts with strong physiological metabolism and low degree of differentiation is significantly better than that of adult tissues and parts.The types and proportions of growth regulators play a key role in bud regeneration.At present,it is generally believed that hormones play a key role in callus development,adventitious buds,somatic embryogenesis and other morphogenesis duri

关 键 词：果树 芽再生 影响因素 干化处理 

分 类 号：S66[农业科学—果树学]