富氢生理盐水对新生SD大鼠高氧肺损伤白介素-8含量及病理的影响  

Effects of hydrogen-rich saline on interleukin-8 and pathology in rats with hyperoxia-induced lung injury

作　　者：吴春友 宋玉环 苏永红[1] Wu Chunyou;Song Yuhuan;Su Yonghong(Department of Comprehensive Pediatrics,Weifang Maternal and Child Health Hospital,Weifang 262700,China)

机构地区：[1]潍坊市妇幼保健院综合儿科

出　　处：《中华临床医师杂志（电子版）》2019年第10期767-770,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：潍坊市科技局资助项目(2017YX073)

摘　　要：目的观察富氢生理盐水对新生SD大鼠高氧肺损伤的预防作用。方法将新生SD大鼠随机分为3组,空气对照组、高氧对照组、高氧+氢水组。高氧对照组、高氧+氢水组大鼠置于氧浓度为90%~95%的氧气箱中,高氧+氢水组每日腹腔注射富氢生理盐水2次(10 ml/kg),空气对照组、高氧对照组每日腹腔注射生理盐水2次(10 ml/kg),高氧暴露后第1、3、7、10天分别测量各组白介素(IL)-8水平,并观察肺部病理切片。结果与空气对照组对比,高氧对照组IL-8含量明显升高,且肺部病理切片出现肺出血、肺泡间隔变薄,并出现大量肺泡融合现象;与高氧对照组比较,高氧+氢水组IL-8含量明显降低,病理图示肺出血、肺泡融合明显减少。结论富氢生理盐水可一定程度减轻高氧对新生SD大鼠的肺部炎症损伤。Objective To observe the effect of hydrogen-rich saline on hyperoxia-induced lung injury in neonatal Sprague-Dawley(SD)rats.Methods Newborn SD rats were randomly divided into three groups:air group,hyperoxia group,and hyperoxia+hydrogen-rich saline group.The hyperoxia group and the hyperoxia+hydrogen-rich saline group were placed in an oxygen chamber with a 90%-95%oxygen concentration.The hyperoxia+hydrogen-rich saline group was injected with hydrogen-rich saline twice a day(10 ml/kg).The air group and hyperoxia group were injected with normal saline twice a day(10 ml/kg).The clinical manifestations of each group were recorded at 1,3,7,and 10 days after birth.At the same time,the alveolar lavage fluid of each group was taken and interleukin-8 was detected by enzyme linked immunosorbent assay(ELISA),and the pathological changes of the lungs were observed.Results Compared with the air group,the content of interleukin-8 in the hyperoxia group was significantly higher.In the hyperoxia group,pulmonary hemorrhage,thinning of alveolar septum,and massive alveolar fusion were observed.Compared with the hyperoxia group,the content of interleukin-8 in the hyperoxia+hydrogen-rich saline group was significantly lower,and pulmonary hemorrhage and pulmonary alveolar fusion were significantly reduced.Conclusion Hydrogen-rich saline can alleviate hyperoxia-induced lung inflammation in neonatal SD rats.

关 键 词：富氢生理盐水 高氧肺损伤 白介素-8 

分 类 号：R722.1[医药卫生—儿科]