烟草BELL基因家族鉴定及其温室条件下的表达模式分析  

Identification of tobacco BELL gene family and their expression patterns in greenhouses

作　　者：宋卫娜 赵森森 武明珠[1] 王根发 罗朝鹏[1] 王晨[1] 李泽锋[1] 杨军[1] 王中[1] SONG Weina;ZHAO Sensen;WU Mingzhu;WANG Genfa;LUO Zhaopeng;WANG Chen;LI Zefeng;YANG Jun;WANG Zhong(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;College of Life Science,Zhengzhou University,Zhengzhou 450001,China;Technology Center,China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]郑州大学生命科学学院,郑州450001 [3]河南中烟工业有限责任公司技术中心,郑州45000

出　　处：《烟草科技》2020年第1期1-11,共11页Tobacco Science & Technology

基　　金：中国烟草总公司郑州烟草研究院院长基金项目“烟草转录因子NtMYB27调控类黄酮合成流向的功能研究”(902017CA0220);河南省自然科学基金项目“NtDREB-1BL1基因调控植物类胡萝卜素含量的分子机制研究”(182300410053)。

摘　　要：BELL基因是植物中普遍存在的一类基因,其编码的同源异型结构域转录因子参与调控了植物叶片、花序等多个组织的发育过程。为明确烟草BELL基因的结构和功能,通过同源序列比对和功能域分析鉴定了普通烟草BELL基因,结合系统进化和基因表达模式分析对烟草BELL基因的功能进行了初步预测。结果表明,从普通烟草基因组中共鉴定出28个NtBELLs基因,分布于15条不同的染色体上。系统进化分析表明,烟草NtBELLs基因可分为4组,每组参与调控烟草不同的发育过程。NtBELLs基因在6个烟草组织中呈现不同的表达模式,且具有明显的组织特异性。干旱和盐胁迫能显著诱导部分NtBELLs基因的表达,表明该基因家族可能参与了植物抵御非生物胁迫的过程。BELL genes,a ubiquitous class of genes in plants and encoded transcription factors with homeobox domains,involve in regulating the development of many plant tissues,such as leaves,inflorescences,etc.To clarify the structure and function of tobacco BELL genes,NtBELL were firstly identified by homology comparison and functional domain analysis.The functions of NtBELL were preliminarily predicted by phylogenetics and gene expression pattern analysis.The results showed that 28 NtBELLs were identified in the genome of Nicotiana tabacum and distributed on 15 chromosomes separately.Phylogenetic analysis indicated that NtBELLs could be divided into four groups and each group participated in regulating different developmental processes.NtBELL had different expression patterns in six tobacco tissues and presented apparent tissue specificity.Drought and salt stress significantly induced the expressions of some NtBELLs,which suggested that BELL gene family might be involved in resisting abiotic stress in plants.

关 键 词：烟草 BEL1-like基因家族 基因结构 系统发育 基因表达 

分 类 号：TS413[农业科学—烟草工业]