DNA条形码技术在中药材鉴定中的应用  

Application of DNA Barcode Technology in Identification of Chinese Medicinal Materials

作　　者：李笑[1] 谭朝阳[1,2] 徐德宏[1] 孟蕾[1] LI Xiao;TAN Zhao-yang;XU De-hong(Lab of Bioengineering,Hunan University of Chinese Medicine,Changsha,Hunan 410208;Lab of Nature and Effectiveness of Chinese Medicine,Hunan University of Chinese Medicine,Changsha,Hunan 410208)

机构地区：[1]湖南中医药大学生物工程实验室,湖南长沙410208 [2]湖南中医药大学中药药性与药效实验室,湖南长沙410208

出　　处：《安徽农业科学》2020年第2期16-19,共4页Journal of Anhui Agricultural Sciences

基　　金：长沙市科技局2018年一般项目(kq1801042);湖湘中药资源保护与利用2011协同创新中心科研项目

摘　　要：DNA条形码技术是一种新兴的分子鉴定方法,它能弥补传统化学方法和形态学方法鉴定中药材的不足之处。通过查阅文献,在分析DNA条形码技术的原理、发展的基础上,对目前一些热门的DNA条形码候选序列ITS、ITS2、psbA-trnh、rbcL、matK展开讨论,重点分析其在植物类和动物类中药材鉴定中的应用,为中药材的鉴定提供新的思路。DNA barcode technology is an emerging molecular identification method that can make up for the shortcomings of traditional chemical methods and morphological methods to identify Chinese herbal medicines.By consulting the literature,on the basis of analyzing the principle and development of DNA barcode technology,some popular DNA barcode candidate sequences ITS,ITS2,psbA-trnh,rbcL,matK are discussed,focusing on its application in the identification of Chinese herbal medicines in plants and animals,and provided new ideas for the identification of Chinese herbal medicines.

关 键 词：DNA条形码 中药材 鉴定 应用 

分 类 号：R282.5[医药卫生—中药学]