种鸡舍采用臭氧水消毒的适宜使用方法研究  

Study on the Suitable Method for Ozone Water Disinfection of Breeding Chicken House

作　　者：段坤 王述柏[2] 初欢欢 张倩 秦志华[2] 周传凤 DUAN Kun;WANG Shu-bai;CHU Huan-huan(Yantai Land Husbandry Co.,Ltd.,Yantai,Shandong 265100;College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong 266109)

机构地区：[1]烟台大地牧业股份有限公司,山东烟台265100 [2]青岛农业大学,山东青岛266109

出　　处：《安徽农业科学》2020年第2期109-112,共4页Journal of Anhui Agricultural Sciences

基　　金：山东省2017年度农业重大应用技术创新项目“肉种鸡精细化健康养殖技术集成与示范”;山东省现代农业产业技术体系家禽产业创新团队建设项目(SDAIT-11-14);2017年山东省农业良种工程(南种北繁)项目(2017LZN046)

摘　　要：[目的]研究臭氧水在种鸡舍的消毒效果及使用方法。[方法]对空舍期鸡舍使用20 mg/L的臭氧水分别消毒15、30、60 min,检测消毒前后菌落数的变化情况;使用臭氧水浸泡水线消毒30 min,观察臭氧水对水线内细菌的杀灭率;每天使用10 mg/L的臭氧水带鸡消毒30 min,连续处理7 d,检测处理前、后鸡舍内菌落总数、氨气、硫化氢含量及种鸡生产性能。[结果]空舍期鸡舍使用20 mg/L的臭氧消毒30和60 min,细菌杀灭率在98%以上,与消毒15 min和甲醛消毒法差异显著(P<0.05);臭氧水对水线消毒30 min,总细菌杀灭率达99.04%,大肠杆菌未检出;臭氧水带鸡消毒对种鸡的生产性能无不良影响(P>0.05),能明显减少舍内的菌落总数,降低空气中氨和硫化氢的含量(P>0.05)。[结论]空舍期种鸡舍和水线使用20 mg/L的臭氧水消毒30 min可以有效杀灭微生物,使用10 mg/L臭氧水每天带鸡喷雾消毒30 min可以有效减少鸡舍内菌落总数和有害气体的产生。[Objective]To study the disinfection effect and ozone water disinfection method of breeding chicken house.[Method]The quantities changes of bacterial colonies in chicken house in the period of empty houses were detected before and after disinfection by using 20 mg/L ozone water for 15,30 and 60 min,respectively.The inactivation rate of bacteria in drinking water pipe was detected after being disinfected by ozone water for 30 min.The breeding chicken in house were disinfected with 10 mg/L ozone water for 30 min every day for 7 days.Then the quantities of bacterial colonies,ammonia gas,hydrogen sulfide content were detected before and after this treatment,and the production performance of breeding chicken were also detected.[Result]The inactivation rates of bacteria in the period of empty houses disinfected by 20 mg/L ozone water for 30 and 60 min respectively were more than 98%,which had significant difference with that in 15 min treatment group and formaldehyde disinfection group(P<0.05).The overall bactericidal rate in drinking water pipe disinfected by ozone water for 30 min was 99.04%,in which E.coli was not detected.The disinfection on breeding chicken in house had no bad influences on the production performance of breeding chicken(P>0.05),but the quantities of bacterial colonies in chicken house obviously decreased,and the contents of ammonia gas and hydrogen sulfide in the atmosphere reduced(P>0.05).[Conclusion]The disinfection with 20 mg/L ozone water for 30 min in breeding chicken house and drinking water pipe in the period of empty houses could effectively kill the microorganisms,and the spraying disinfection with 10 mg/L ozone water for 30 min daily could effectively reduce the total number of colonies and harmful gases in the chicken house.

关 键 词：种鸡 臭氧水 消毒 

分 类 号：S831.4[农业科学—畜牧学]