番石榴叶有机溶剂提取物清除过氧自由基能力及抑制酪氨酸酶活力研究  

Study on Scavenging Peroxy Radical Activity and Inhibitory Effect on Tyrosinase of Guava Leaves Extract

作　　者：叶伟 YE Wei(Lingnan Normal University,Zhanjiang,Guangdong 524048)

机构地区：[1]岭南师范学院

出　　处：《安徽农业科学》2020年第2期186-188,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究番石榴叶不同有机溶剂提取物对自由基清除能力以及对酪氨酸酶活力的抑制作用,可为番石榴叶在抗氧化抗衰老方面的应用奠定基础。[方法]采用乙醇、乙酸乙酯、石油醚和丙酮4种不同有机溶剂并采取超声、浸提及萃取的方法提取番石榴叶粗提物。采用邻苯三酚自氧化法和L-DOPA法分别测定粗提物对过氧自由基的清除作用和酪氨酸酶活性的抑制作用。[结果]乙醇、丙酮和乙酸乙酯番石榴叶提取物清除过氧自由基的IC 50值分别为47.1、35.5和21.2μg/mL;而丙酮提取物和乙酸乙酯提取物抑制酪氨酸酶活性的IC 50值分别为165.3和115.9μg/mL。[结论]番石榴叶提取物具有开发作为抗氧化抗衰老药物以及酪氨酸酶抑制剂的应用前景。[Objective]The effects of different organic solvent extracts on free radical scavenging and tyrosinase activity of guava leaves were studied.[Method]The crude extract of guava leaves was extracted by ultrasonic,immersion and extraction methods using four different organic solvents(ethanol,ethyl acetate,petroleum ether and acetone).The scavenging effect of peroxy free radicals and inhibition of tyrosinase activity of crude extracts were determined by catechol autooxidation method and l-dopa method respectively.[Result]The IC 50 values of ethanol,acetone and ethyl acetate guava leaf extracts for scavenging peroxyl radicals were 47.1,35.5 and 21.2μg/mL,respectively;but the IC 50 values for the inhibition of tyrosinase activity by acetone extract and ethyl acetate extract were 165.3 and 115.9μg/mL,respectively.[Conclusion]The extract of guava leaf has the prospect of developing as antioxidant and anti-aging drug and tyrosinase inhibitor.

关 键 词：番石榴叶 粗提物 过氧自由基 酪氨酸酶活性 

分 类 号：R284[医药卫生—中药学]