细胞氧化应激过程中几种关键分子的SERS检测方法  

SERS detection methods for several key molecules in the oxidative stress process of cells

作　　者：岳静[1] 申燕婷 王佳琪[1] 徐蔚青[1] 徐抒平[1] YUE Jing;SHEN Yanting;WANG Jiaqi;XU Weiqing;XU Shuping(State Key Laboratory of Supramolecular Structure and Materials,Institute of Theoretical Chemistry,College of Chemistry,Jilin University,Changchun,130012)

机构地区：[1]吉林大学超分子结构与材料国家重点实验室理论化学研究所化学学院

出　　处：《光散射学报》2019年第4期317-325,共9页The Journal of Light Scattering

基　　金：家自然科学基金(21573092,21573087,21873039)

摘　　要：细胞的氧化过程与癌症、心血管疾病、神经退行性疾病、糖尿病等多种疾病的发展密切有关。针对氧化反应发生,细胞产生应激反应。这一系列刺激响应过程中会产生一些关键性分子,例如活性氧簇分子(ROS)等。对这些关键分子的分析检测不仅可以有助于理解这些分子在过程中扮演的重要角色,更是对疾病的发生和发展规律有深入的理解。本文重点汇总了分析和检测细胞内氧化应激关键分子所采用的表面增强拉曼光谱(SERS)技术,从SERS方法的设计策略出发,对现有方法归类总结,枚举了多项中国同行的研究工作,并展望了其未来发展方向。Oxidative stress is associated with the development of various diseases including cancer,cardiovascular disease,neurodegenerative diseases and diabetes mellitus.In response to these oxidation reactions,cells start stress responses.A lot of the key molecules,e.g reactive oxygen species molecules(ROS),would be produced during oxidative stress and they play an extremely important role.The analysis and detection of these key molecules can not only help to understand the important roles of these molecules in the oxidative stress process,but also can have a deeper understanding of the occurrence and development of diseases.In this review,we focused on summarizing the surface-enhanced Raman spectroscopic techniques that realize the analyses and detections of the key molecules of oxidative stress in cells.Starting from the designed strategies of SERS methods,we classified and summarized the existing methods,enumerated a number of research work of Chinese scientists,and displayed the outlook for this field.

关 键 词：氧化应激 细胞内检测 表面增强拉曼 SERS标记 活性氧簇 

分 类 号：O65[理学—分析化学]