基于拉曼光谱的快速细菌诊断  

Rapid Bacterial Diagnosis by Raman Spectroscopy

作　　者：杨凯 李弘哲 朱永官[1] 崔丽[1] YANG Kai;LI Hongzhe;ZHU Yongguan;CUI Li(Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021;University of Chinese Academy of Sciences,Beijing 100049)

机构地区：[1]中国科学院城市环境研究所,城市环境与健康重点实验室,厦门361021 [2]中国科学院大学,北京100049

出　　处：《光散射学报》2019年第4期336-345,共10页The Journal of Light Scattering

基　　金：国家自然科学基金优秀青年科学基金(21922608);国家自然科学基金水圈重大研究计划(91851101);国家自然科学基金面上项目(21777154);中国科学院基础前沿科学研究计划“从0到1”原始创新项目(ZDBS-LY-DQC027)

摘　　要：致病菌一直被认为是对公共卫生最严重的威胁之一。随着抗生素耐药性在全球范围的出现和蔓延,耐药致病菌的健康威胁进一步加剧。对致病菌的快速识别及其抗生素药物敏感性的快速检测,对高效诊断和治疗相关疾病至关重要。拉曼光谱可以提供致病菌的指纹图谱信息,是一种非常有前景的细菌快速鉴定及抗生素药敏检测工具。本文综述了基于单细胞拉曼光谱和表面增强拉曼光谱进行致病菌快速诊断的最新研究进展,包括细菌快速鉴定、细菌药敏性快速检测、临床样品前处理与细菌分离方法、谱图分析的化学计量学方法等,并对该领域未来的发展进行了展望。Pathogenic bacteria have long been regarded as one of the most serious threats to public health.With the globally growing antibiotic resistance,health threat from antibiotic resistant pathogens is dramatically increasing.Rapid identification of pathogenic bacteria and antibiotic susceptibility testing(AST)are essential for efficient diagnosis and treatment.Raman spectroscopy is a very promising method capable of providing fingerprint information of pathogenic bacteria for both identification and AST.Here,we reviewed the recent achievements of utilizing both single-cell Raman and surface-enhanced Raman spectroscopy for rapid bacterial diagnosis,including rapid bacterial identification and AST,pretreatment of clinical samples for bacteria separation,and chemometric methods for spectral analysis.Perspective on future development and challenges in Raman methodology was also provided.

关 键 词：单细胞拉曼光谱 表面增强拉曼 细菌快速鉴定 快速抗生素药敏检测 

分 类 号：O657.37[理学—分析化学] R446.5[理学—化学]