黄芪破壁饮片4种黄酮类成分分析及重金属含量测定  

Analysis of four flavonoids and determination of heavy metal content in ultrafine granular power of Radix Astragali

作　　者：王艳 郑夏生[3] 李文康 成金乐 WANG Yan;ZHENG Xiasheng;LI Wenkang;CHENG Jinle(Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine,Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Chinese Medicine),Ministry of Education,Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines,Guangzhou,Guangdong 510006,China;Key Laboratory of Technologies and Applications of Ultrafine Granular Powder of Chinese Medica,State Administration of Traditional Chinese Medicine,ZEUS Pharmaceutical Group,Zhongshan,Guangdong 528437,China;Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong 510006,China)

机构地区：[1]广州中医药大学中药资源科学与工程研究中心、岭南中药资源教育部重点实验室(广州中医药大学)、国家中成药工程技术研究中心南药研发实验室,广东广州510006 [2]国家中医药管理局中药破壁饮片技术与应用重点研究室,中山市中智药业集团有限公司,广东中山528437 [3]中医药数理工程研究院,广州中医药大学,广东广州510006

出　　处：《安徽医药》2020年第2期247-251,共5页Anhui Medical and Pharmaceutical Journal

基　　金：广东省科学技术厅科技计划项目(2014B010108013)

摘　　要：目的评价黄芪破壁饮片的有效性和安全性。方法利用高效液相色谱法建立一种可以同时测定黄芪破壁饮片中4种黄酮类成分(毛蕊异黄酮葡萄糖苷、刺芒柄花苷、毛蕊异黄酮、芒柄花素)含量的方法,同时采用电感耦合等离子体质谱法对黄芪破壁饮片中5种重金属(Cd、Cu、Pb、Hg、As)的含量进行监测。结果黄芪破壁饮片中4种黄酮类成分的线性关系良好(r≥0.999 5),加样回收率在98.6%~106.3%之间,方法的重复性和稳定性良好,表明该方法可用于黄芪破壁饮片的4种黄酮类成分含量的同时监测,可为黄芪质量控制提供更全面的参考;10批黄芪破壁饮片中5种重金属[镉(Cd)、铜(Cu)、铅(Pb)、汞(Hg)、砷(As)]的含量均符合相关限量规定。结论同时进行4种黄酮类成分分析及重金属含量测定,可以从有效物质基础和有害元素多个角度综合评价黄芪的质量和安全性。Objective To evaluate the efficacy and safety of Ultrafine Granular Power of Radix Astragali.Methods This experiment set up a method which can simultaneously determinate the contents of four flavonoids(Calycosin-7-O-glucoside,Ononin,Calycosin and Formononetion)in Ultrafine Granular Power of Radix Astragali.At the same time,Inductively Coupled Plasma Mass Spectrometry was used to examine the contents of five heavy metal residuals(Cd,Cu,Pb,Hg,and As).Results Under the experimental conditions of our study,the four flavonoids had good linear relationship(r≥0.999 5),and the recovery rate of four components were within 98.6% and 106.3%.The repeatability and stability of the method was considerable as well.The results show that this method could be applied to the determination of four flavonoids in Ultrafine Granular Power of Radix Astragali simultaneously and could provide more comprehensive reference for quality control of Radix Astragali.Besides,The contents of five heavy metal residuals(Cd,Cu,Pb,Hg,As)in the ten batches of Radix Astragali were in compliance with relevant regulations.Conclusion The quality and safety of Radix Astragali can be evaluated comprehensively from the multiple perspectives of effective material basis and harmful elements by combining the content of active ingredients with the safety indicators such as heavy metals.

关 键 词：黄芪 黄酮类 饮片 色谱法 高压液相 重金属残留 

分 类 号：R284.1[医药卫生—中药学]