菠萝PEPC基因家族生物信息学分析  

Bioinformatics Analysis of PEPC Gene Family in Pineapple

作　　者：马海洋[1,2] 赵秋芳 陈曙[1,3] 石伟琦 冼皑敏[1,2] MA Haiyang;ZHAO Qiufang;CHEN Shu;SHI Weiqi;XIAN Aimin(South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091,China;Hainan Key Laboratory of Tropical Crops Nutrition,Zhanjiang,Guangdong 524091,China;Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs,Zhanjiang,Guangdong 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]海南省热带作物营养重点实验室,广东湛江524091 [3]农业农村部热带果树生物学重点实验室,广东湛江524091

出　　处：《热带作物学报》2020年第1期97-103,共7页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院基本科研业务费专项(No.1630062019026,No.1630062018012)

摘　　要：磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)在C4和景天酸代谢(CAM)光合途径吸收CO2过程中发挥重要作用,并参与各种非光合作用过程,包括果实成熟、气孔开放、碳氮相互作用、种子形成和萌发以及调节植物对逆境胁迫的耐受性。菠萝为典型的景天酸代谢途径(CAM)植物,为了解磷酸烯醇式丙酮酸羧化酶(PEPC)在菠萝景天酸代谢途径(CAM)中的作用,本研究鉴定到3个菠萝PEPC基因,按照基因描述命名为AcPEPC1、AcPEPC3和AcPEPC4,进化树分析结果AcPEPC1和AcPEPC3为植物型PEPC(PTPCs)蛋白,序列同源性较高且基因结构、保守结构域及保守基序均一致。AcPEPC4为细菌型PEPC(BTPCs)蛋白,与AcPEPC1/AcPEPC3间的序列同源性低。组织表达分析表明AcPEPC1菠萝叶片表达量较高,而AcPEPC3和AcPEPC4在菠萝花和果实表达量较高。Phosphoenolpyruvate carboxylase(PEPC)plays an important role in assimilating atmospheric CO2 during C4 and Crassulacean acid metabolism(CAM)photosynthesis,and participates in various nonphotosynthetic processes,including fruit ripening,stomatal opening,supporting carbon-nitrogen interactions,seed formation and germination,and regulation of plant tolerance to stresses.Pineapple is a typical CAM plant.In order to understand the function of PEPC gene in pineapple CAM photosynthesis pathway,three PEPC genes were identified in pineapple and nominated as AcPEPC1/AcPEPC3/AcPEPC4 according to the gene description.Phylogenetic analysis showed that AcPEPC1 and AcPEPC3 protein belonged to the plant type PEPC(PTPCs)subfamily,which had high sequence homology.Gene structure,conserved domain and conserved motif of AcPEPC1 and AcPEPC3 had high similarity.AcPEPC4 belonged to the bacterial type of PEPC(BTPCs)subfamily which had low sequence homology with AcPEPC1/AcPEPC3.Different tissues expression assay showed that the expression of AcPEPC1 was high in leaves,and AcPEPC3 and AcPEPC4 was highly expressed in flowers and fruits.

关 键 词：菠萝 磷酸烯醇式丙酮酸羧化酶(PEPC) 基因进化 基因表达谱 

分 类 号：S668.3[农业科学—果树学]