丝胶对2型糖尿病大鼠肝脏TRB3的调节作用  

Regulatory effects of sericin on liver TRB3 in rats of type 2 diabetes mellitus

作　　者：刘东慧[1] 付文亮[1] 付秀美[1] 宋成军[1] 陈志宏[1] LIU Dong-Hui;FU Wen-Liang;FU Xiu-Mei(Department of Human Anatomy,Chengde Medical University,Chengde 067000,Hebei,China)

机构地区：[1]承德医学院人体解剖学教研室

出　　处：《中国老年学杂志》2020年第2期366-369,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81441133);河北省自然科学基金资助项目(H2013406096);承德医学院自然科学青年基金项目(201819)

摘　　要：目的研究2型糖尿病大鼠肝脏肝细胞Tribbles同源蛋白(TRB)3的表达变化及丝胶的调节作用。方法选择36只SPF级雄性SD大鼠作为研究对象,随机分为正常对照组(12只)和造模组(24只)。造模组大鼠以高脂高糖饲料喂养+腹腔注射链脲佐菌素的方法构建2型糖尿病大鼠模型,将成模的大鼠随机分为模型组和丝胶治疗组,每组12只,丝胶治疗组大鼠给予丝胶2.4 g/(kg·d)、其余组大鼠给予等体积生理盐水连续灌胃35 d。给药结束,取血液标本采用葡萄糖氧化酶法检测各组大鼠的空腹血糖;取肝脏组织采用SP免疫组织化学法、免疫印迹法和Real Time Q-PCR法分别检测各组大鼠肝细胞TRB3蛋白和mRNA的表达。结果与模型组大鼠相比,丝胶治疗组大鼠的空腹血糖水平、肝细胞TRB3蛋白和mRNA的表达明显降低(tSP免疫组织化学法=-2.32,t免疫印迹法=-4.89,t实时定量PCR法=-3.08,P<0.05)。结论丝胶降低血糖的作用,可能通过下调肝脏TRB3的表达进而降低对胰岛素磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)信号通路中Akt的抑制作用来实现。Objective To investigate the expression of Tribbles-related protein 3(TRB3)in liver cells of type 2 diabetes mellitus rats and the regulatory effects of sericin.Methods Thirty-six specific pathogen free(SPF)male SD rats were selected as the study subjects and randomly divided into the normal control group(12 rats)and the model group(24 rats).Rats in the model group were fed with high-fat and high-sugar diet and intraperitoneal injection streptozotocin(STZ)to construct the model of type 2 diabetes mellitus.After successfully building the model,the rats were randomly divided into model group and sericin treatment group,with 12 rats in each group.Rats in the sericin treatment group were given sericin 2.4 g/(kg·d)for 35 days,and rats in the other groups were given constant volume normal saline for 35 days.The glucose oxidase method was used to detect the fasting blood glucose of rats in each group.The expressions of TRB3 protein and mRNA in liver were detected by SP immunohistochemical staining,Western blot and Real time Q-PCR method.Results Compared with the rats of model group,the level of fasting blood glucose,the expression of TRB3 protein and mRNA in rats liver of sericin treatment group were significantly reduced(P<0.05).Conclusions The effect of sericin on reducing blood glucose might be achieved by down-regulating the expression of liver TRB3 and reducing the inhibition of Akt in the insulin phosphatidylinositol-3-kinase/protein kinase B(PI3 K/Akt)signaling pathway.

关 键 词：丝胶 2型糖尿病 肝脏 肝细胞Tribbles同源蛋白3 

分 类 号：R587.1[医药卫生—内分泌]