纳米铁氰化钴电化学传感器检测细胞释放的超氧阴离子  

Electrochemical Detection of Superoxide Anion Released from Living Cells Using Nano Cobalt Ferricyanide

作　　者：崔敏 温晓芳 任聚杰 张聪 李娜 韩元元 余旭东 籍雪平 CUI Min;WEN Xiao-Fang;REN Ju-Jie;ZHANG Cong;LI Na;HAN Yuan-Yuan;YU Xu-Dong;JI Xue-Ping(Department of Chemistry,School of Sciences,Hebei University of Science and Technology,Shijiazhuang 050018,China;Department of Medical Chemistry,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北科技大学理学院化学系,石家庄050018 [2]河北医科大学药学院,石家庄050017

出　　处：《分析化学》2020年第2期224-232,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.81872669);河北省高等学校科学技术研究项目(Nos.ZD2018037,QN2019140)资助~~

摘　　要：采用共沉淀方法制备的铁氰化钴(Co3[Fe(CN)6]2)和Hummers法制备的还原氧化石墨烯(RGO)共沉积修饰玻碳电极(GCE),制备了无酶型超氧阴离子(O2^·-)电化学传感器(Co3[Fe(CN)6]2-RGO/GCE)。采用扫描电子显微镜(SEM)、X射线衍射(XRD)、电化学交流阻抗法(EIS)、循环伏安法(CV)等研究了复合材料Co3[Fe(CN)6]2-RGO的特征及其对水中O2^·-的电化学检测的可行性,分析了Co3[Fe(CN)6]2-RGO/GCE检测O2^·-的作用机理。传感器对O2^·-浓度的检测范围为0.5~30μmol/L,灵敏度为91.8μA L/(μmol cm^2),检出限为0.071μmol/L(S/N=3),而且具有良好的选择性,H2O2、O2及其它体内电化学活性物质均不干扰O2^·-的测定,成功地应用于MCF-7细胞中释放的O2^·-的电化学检测。此传感器在病理和生物学领域定量测定O2^·-方面具有潜在的应用价值。A non-enzymatic superoxide anion(O2^·-)electrochemical sensor(Co3[Fe(CN)6]2-RGO/GCE)was fabricated by co-depositing cobalt ferricyanide(Co3[Fe(CN)6]2)prepared by coprecipitation method and reduced graphene oxide(RGO)prepared by Hummers method on a glassy carbon electrode(GCE).The characteristics of Co3[Fe(CN)6]2-RGO nanocomposites and the possibility for electrochemical detection of O2^·- were studied using scanning electron microscopy(SEM),X-ray diffraction(XRD),electrochemical impedance spectroscopy(EIS)and cyclic voltammetry(CV).The mechanism of Co3[Fe(CN)6]2-RGO for O2^·- detection was investigated in this work.The sensor had a sensitivity of 91.8μA L/(μmol cm^2)and a detection limit of 0.071μmol/L(S/N=3).Besides,it had excellent selectivity and could avoid the interference of hydrogen peroxide,oxygen and other electrochemical active substances in vivo.It was successfully applied to the electrochemical detection of superoxide anions in stimulated MCF-7 cells.The sensor had potential application for quantifying superoxide anions in the field of pathology and biology.

关 键 词：电化学传感器 超氧阴离子 铁氰化钴 还原氧化石墨烯 MCF-7细胞 

分 类 号：R446.1[医药卫生—诊断学] O657.1[医药卫生—临床医学]