波谱形变定量理论结合核壳型颗粒用于生物样本中6-硫鸟嘌呤的检测  

Quantification of 6-Thioguanine in Biological Samples by Spectral Shape Deformation Quantitative Theory Combined with Core-shell Nanoparticles

作　　者：王颖琦 陈瑶 陈增萍[1] WANG Ying-Qi;CHEN Yao;CHEN Zeng-Ping(State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China;Hunan Key Lab of Biomedical Materials and Devices,College of Life Sciences and Chemistry,Hunan University of Technology,Zhuzhou 412008,China)

机构地区：[1]化学生物传感与计量学国家重点实验室,湖南大学化学化工学院,长沙410082 [2]湖南省生物医用纳米材料与器件重点实验室,湖南工业大学生命科学和化学学院,株洲412008

出　　处：《分析化学》2020年第2期269-274,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21705044,21775038);湖南省自然科学基金项目(No.2018JJ3114)资助~~

摘　　要：制备了Au-core@4-巯基苯甲酸@Ag-shell核壳型纳米颗粒,结合波谱形变定量分析理论,采用表面增强拉曼光谱(SERS)定量分析了尿液和红细胞样本中6-硫鸟嘌呤(6-TG)。实验结果表明,核壳型纳米颗粒和波谱形变定量分析理论相结合可以有效消除SERS增强基底表面的“热点”数目及其分布等因素的变化对SERS信号的乘子效应影响,从而实现尿液和红细胞样本中6-TG的灵敏、准确定量分析。本方法对6-TG的检出限(3σ)和定量限(10σ)分别为1 nmol/L和3 nmol/L,加标回收率在95.6%~106.7%之间。相较于传统的6-TG分析方法(如高效液相色谱法),本方法具有简单、灵敏、快速等优点,为复杂体系中6-TG的常规定量分析提供了参考。The core-shell nanoparticles(i.e.,Au-core@4-mercaptobenzoic acid@Ag-shell)were combined with spectral shape deformation(SSD)quantitative model for quantification of 6-thioguanine(6-TG)in urine and red blood cell lysate samples.Experimental results showed that the combination of core-shell nanoparticles with SSD model could effectively remove the detrimental multiplicative effects on samples'surface enhanced Raman spectroscopy(SERS)signals caused by the variations of the number of“hot spots”and their distribution near/on the surfaces of SERS enhancing substrates,and hence realized the accurate quantitative determination of 6-TG in urine and red blood cell lysate samples with recovery of 95.6%-106.7%.The limit of detection(3σ)and limit of quantification(10σ)for 6-TG were estimated to be about 1 and 3 nmol/L,respectively.Compared with conventional methods for analysis of 6-TG such as high performance liquid chromatography(HPLC),the proposed method had many advantages such as simplicity,rapidity and high sensitivity,and had great potential in routine quantitative analysis of 6-TG in complex samples.

关 键 词：表面增强拉曼光谱 6-硫鸟嘌呤 核壳型纳米颗粒 波谱形变定量分析理论 

分 类 号：R446.1[医药卫生—诊断学] O657.37[医药卫生—临床医学]