五指山猪背最长肌和半腱肌DNA甲基化检测分析  

Analysis on DNA Methylation of Longissimus Dorsi and Semitendinosus in Wuzhishan Pig

作　　者：晁哲[1,2] 孙瑞萍 刘海隆[1,2] 邢漫萍 郑心力 CHAO Zhe;SUN Ruiping;LIU Hailong;XING Manping;ZHENG Xinli(Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China;Hainan Provincial Engineering Research Center for Livestock and Poultry Cultivation,Haikou 571100,China)

机构地区：[1]海南省农业科学院畜牧兽医研究所,海南海口571100 [2]海南省家畜家禽工程技术研究中心,海口571100

出　　处：《养猪》2020年第1期44-46,共3页Swine Production

基　　金：国家自然科学基金项目（31560628）;海南自然科学基金创新研究团队项目（2017CXTD013）

摘　　要：DNA甲基化作为真核生物基因组重要的表观遗传学修饰,对生物体基因的表达有重要的调控作用。为了研究五指山猪肌肉组织基因组DNA的甲基化程度,试验利用MSAP技术,使用筛选的10对引物扩增,检测五指山猪背最长肌和半腱肌的甲基化程度。结果显示:1月龄、2月龄、4月龄五指山猪背最长肌的平均甲基化率分别是44.78%、41.58%、38.92%;1月龄、2月龄、4月龄五指山猪半腱肌的平均甲基化率分别是41.70%、39.39%、38.81%。研究结果为五指山猪生长发育规律、系统选育及矮小机制等方面的研究提供表观遗传学依据。As an important epigenetic modification factor in eukaryotic genomes,DNA methylation plays a vital role in regulation of gene expression.The study is to determine the genome-wide DNA methylation level in skeletal muscle of Wuzhishan pig.We used methylation sensitive amplification polymorphism(MSAP)method and 10 pairs of selective primers to assess the extent and pattern of cytosine methylation in longissimus dorsi and semitendinosus.The results showed that the percentage methylation of longissimus dorsi at 1 m,2 m and 4 m was 44.78%,41.58%,38.92%,respectively.The incidence of DNA methylation in semitendinosus reached 41.70%,39.39%,38.81%at 1 m,2 m and 4 m,respectively.This study provides some useful epigenetic basis principles for growth and development,system selection,short mechanism of Wuzhishan pig.

关 键 词：五指山猪 肌肉 DNA 甲基化 

分 类 号：S828.8[农业科学—畜牧学]