不同地方猪种背部皮下脂肪中miR-206差异表达研究  

Differential Expression Analysis of miR-206 in Subcutaneous Adipose Tissue of Different Native Pig Breeds

作　　者：陶璇[1] 顾以韧[1] 杨雪梅[1] 梁艳[1] 何志平[1] 钟志君 杨跃奎[1] 曾凯[1] 陈晓晖[1] 王言 龚建军[1] 应三成[1] 吕学斌[1] TAO Xuan;GU Yiren;YANG Xuemei;LIANG Yan;HE Zhiping;ZHONG Zhijun;YANG Yuekui;ZENG Kai;CHEN Xiaohui;WANG Yan;GONG Jianjun;YING Sancheng;LV Xuebin(Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy,Chengdu 610066,China)

机构地区：[1]四川省畜牧科学研究院动物遗传育种四川省重点实验室

出　　处：《养猪》2020年第1期47-48,共2页Swine Production

基　　金：四川省青年科技基金（2017JQ0011,2016JQ0041）;国家生猪产业技术体系项目（CARS-35）;四川省科技支撑计划项目（2016NYZ0042,2016NYZ0026,2016NZ0108）;四川省重大科技专项（2017NZDZX0002）;四川省重点研发项目（2018NZ0150,2019YFN0035）;四川省财政运行专项（SASA2014CZYX001）;现代农业产业技术体系四川省生猪创新团队（SCCXTD-001,SCCXTD-005,SCCXTD-008）;四川省科研院所基本科研项目（SASA2018A02）

摘　　要：试验采用荧光定量PCR技术(QRT-PCR)对6个四川省地方猪种背部皮下脂肪中miR-206的差异表达情况进行测定和分析。结果表明:藏猪miR-206相对表达量最高,成华猪次之,青峪猪最低;藏猪miR-206相对表达量显著高于内江猪(P<0.05),极显著高于丫杈猪和青峪猪(P<0.01)。Quantitative real-time PCR(QRT-PCR)was used in this experiment for the determination and analysis of differential expression of miR-206 in back subcutaneous adipose tissues of six nativepig breeds in Sichuan province.The results showed that the expression level of miR-206 in Tibet pigs was the highest,which was significantly(P<0.05)higher than that in Neijiang pigs,also extremely significantly(P<0.01)higher than that in Yacha pigs and Neijiang pigs.

关 键 词：猪 miR-206 荧光定量PCR 背部皮下脂肪 

分 类 号：S828[农业科学—畜牧学]