反相高效液相色谱法测定血清中阿帕替尼的浓度  

Determination of Apatinib in Serum by RP-HPLC

作　　者：范芳[1] 余炜[1] 刘捷[2] 陈玲[2] 林榕波[2] FAN Fang;YU Wei;LIU Jie;CHEN Lin;LIN Rong-bo(Department of pharmacology,Fujian Provincial Tumor Hospital,Fuzhou 350014 China;Department of internal Medicine,Fujian Provincial Tumor Hospital,Fuzhou 350014 China)

机构地区：[1]福建省肿瘤医院、福建医科大学附属肿瘤医院,药剂科,福建福州350014 [2]福建省肿瘤医院、福建医科大学附属肿瘤医院内科,福建福州350014

出　　处：《海峡药学》2020年第1期52-54,共3页Strait Pharmaceutical Journal

基　　金：福建省卫计委计划课题中青年骨干人才培养项目(项目号:2017-ZQN-17);福建省医学创新课题(项目号:2016-CX-12);北京市希思临床肿瘤学研究基金会(项目号:Y-HR2016-055)

摘　　要：目的建立简单高效的阿帕替尼反相高效液相色谱测定法。方法血清中阿帕替尼用3mL乙醚-石油醚(10∶1)单步提取;色谱柱:Symmetry RP18(5μm,4.6mm×250mm,waters),流动相:乙腈-0.2%TFA水溶液(36∶64)。检测波长:阿帕替尼260nm,内标卡马西平286nm。结果阿帕替尼在0.2~8μg·mL^-1范围内呈良好的线性关系(r=0.9991)。结论此方法简便快速,可用于阿帕替尼血药浓度监测。OBJECTIVE A simple extraction of apatinib in serum and a method of HPLC were developed.METHODS Apatinib was extracted from serum by 3mL ethyl ether:petroleum ether(10∶1)and analyzed in a column of Symmetry RP 18(5μm,4.6mm×250mm,waters)with a mobile buffer of acetonitrile-0.2%TFA(36∶64).RESULTS The linear of apatinib was excellent within the range of 0.2-8μg·mL^-1(r=0.9991).CONCLUSION The method is simple,rapid and applied to the therapeutic drug monitoring of apatinib.

关 键 词：反相高效液相色谱法 阿帕替尼 血药浓度监测 

分 类 号：R969.1[医药卫生—药理学]