猪伪狂犬病病毒基因缺失灭活疫苗（LA-A株）的最小免疫剂量和制品保存期的研究  

DETERMINATION OF THE MINIMUM IMMUNE DOSE AND PRESERVATION PERIOD OF INACTIVATED VACCINE OF PSEUDORABIES VIRUS GENE DELETION STRAIN LA-A

作　　者：乔永峰[1,2,3,4] 郭容利 宋增财[1,2,3,4] 刘娅梅 许梦微[1,3,4] 郑亚婷 陈赛赛[1,3,4] 王志胜 张传健 侯继波 范红结[2] 王继春 QIAO Yong-feng;GUO Rong-li;SONG Zeng-cai;LIU Ya-mei;XU Meng-wei;ZHENG Ya-ting;CHEN Sai-sai;WANG Zhi-sheng;ZHANG Chuanjian;HOU Ji-bo;FAN Hong-jie;WANG Ji-chun(Institute of Veterinary Immunology&Engineering,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Nanjing Agricultural University,Nanjing 210095,China;National Research Center of Engineering and Technology for Veterinary Biologicals,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Hangzhou 225009,China;Institute of Veterinary Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院动物免疫工程研究所,南京210014 [2]南京农业大学动物医学院,南京210095 [3]江苏省农业科学院国家兽用生物制品工程技术研究中心,南京210014 [4]江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009 [5]江苏省农业科学院兽医研究所,南京210014

出　　处：《中国动物传染病学报》2020年第1期56-62,共7页Chinese Journal of Animal Infectious Diseases

基　　金：农业公益性行业科研专项经费项目(201403051);江苏省农业科技自主创新资金(CX(18)3020)

摘　　要：本研究将伪狂犬病病毒(Pseudorabies virus,PRV)变异株(PRV AH02LA株)的gE基因缺失株(LA-A株)接种BHK-21细胞,经纯悬浮培养制备抗原,甲醛灭活后制备油乳剂灭活疫苗,并确定该灭活疫苗的最小免疫剂量和效力检验方法,以及在2~8℃保存期。结果显示:猪伪狂犬病病毒基因缺失灭活疫苗(LA-A株)的效力检验方法为以2.0 mL(抗原含量108.20TCID50)接种4~5周龄PRV阴性健康仔猪,颈部肌肉注射,间隔28 d以相同剂量和方法加强免疫,加强免疫后第21 d,免疫猪血清PRV抗体中和指数应不低于10000,攻毒保护率应不低于80%;最小免疫剂量为1.0 mL(抗原含量107.90 TCID50);制品保存期:在2~8℃保存期为18个月。该研究结果为新型疫苗的研制提供了重要的试验依据。In this study,suspension cultures of BHK-21 cells were inoculated with a gE gene-deleted strain LA-A of Pseudorabies virus(PRV)that derived from an emerging PRV variant strain AH02 LA.The harvested viral fluid was inactivated by formaldehyde and formulated into water-in-oil emulsion.This inactivated oil adjuvanted LA-A vaccine was tested for its minimum immunization dosage in pigs and storage stability at 2-8℃.Healthy PRV-antibody-negative piglets at age of 4-5 weeks old were intramuscularly injected twice with 2.0 mL of the LA-A vaccine produced from viral fluid(108.20 TCID50)at an interval of 28 days.The vaccinated pigs were challenged with virulent AH02 LA strain 21 days post the booster vaccination.The efficacy of the LA-A vaccine met the satisfactory requirements including serum PRV antibody neutralization index at no less than 10000 and the protection rate at no lower than 4/5 pigs vaccinated/challenged.Accordingly,the minimal immunization dosage of the LA-A vaccine was determined as 1.0 mL prepared from viral fluid containing 107.90 TCID50.The efficacy of this LA-A vaccine was tested satisfactory for 21 months stored at 2-8℃.Therefore,the preservation period of this LA-A vaccine was determined as 18 months.

关 键 词：猪伪狂犬病 灭活疫苗 最小免疫剂量 保存期 

分 类 号：S852.659[农业科学—基础兽医学]