lmo2672基因缺失对LM在不同环境下生物被膜形成的影响  

Effects of lmo2672 Deficiency on Biofilm Formation of Listeria monocytogenes under Different Environments

作　　者：李杰[1] 孟庆玲[1] 乔军[1] 伍晔晖 王熙凤 王立霞[1] 才学鹏[2] LI Jie;MENG Qingling;QIAO Jun;WU Yehui;WANG Xifeng;WANG Lixia;CAI Xuepeng(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]中国农业科学院兰州兽医研究所,兰州730046

出　　处：《西北农业学报》2020年第1期150-156,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家国际科技合作专项(2013DFR31310);国家自然科学基金(31360596,30960274)~~

摘　　要：旨在了解AraC家族转录调控因子lmo2672对单核增生李斯特菌(LM)生物被膜(BF)形成的影响。根据同源重组技术构建LM-△lmo2672基因缺失株,利用结晶紫染色法比较LM EGD-e和LM-△lmo2672在不同温度、渗透压、H 2O 2和胆酸盐环境中BF形成量的差异;通过qRT-PCR分析上述不同应激环境中BF相关基因相对转录水平的变化。研究结果证实:与LM EGD-e相比,LM-△lmo2672在4℃,30℃,5%NaCl,8%NaCl,5 mmol/L H 2O 2和0.3%胆酸盐培养环境中BF形成量显著降低;在37℃LM-△lmo2672 BF形成能力极显著降低;在不同应激环境中,LM-△lmo2672 BF形成相关基因的表达量均出现不同程度下降。研究结果表明,lmo2672基因在LM BF形成过程中发挥重要作用。In order to explore the function of AraC family regulator lmo2672 on the biofilm formation of LM,△lmo2672 deletion strain was constructed by using homologous recombination technique.We monitored their biofilm formation at different temperatures,osmotic pressures,media containing H 2O 2 and,bile salts we also explored the influences of lmo2672 deletion on the transcription of genes related to biofilm of LM using qRT-PCR.The results showed that compared with LM EGD-e,the biofilm biomass of lmo2672 deletion mutant strain significantly decreased under 4℃,30℃,5%NaCl,8%NaCl,5 mmol/L H2O2 and 0.3%bile salts stresses,and biofilm formation of the lmo2672 deletion mutant was significantly compromised under 37℃.Furthermore,in different environmental stresses,the expressions of the biofilm-related genes decreased to different degrees in LM-Δlmo2672.Therefore,these findings suggested that the lmo2672 of AraC family plays important roles in the Listeria monocytogenes biofilm formation.

关 键 词：单核增生李斯特菌 AraC家族 lmo2672基因 生物被膜 

分 类 号：Q93[生物学—微生物学]