补肾益气活血方对胚胎着床障碍大鼠子宫内膜孕激素受体和信号传导与活化转录因子3表达的影响  

Effect of Bushen Yiqi Huoxue recipe on the expression of PR and STAT3 in rats with embryo implantation dysfunction

作　　者：钟志艳 黄冬梅[1] 黄光英[1] ZHONG Zhiyan;HUANG Dongmei;HUANG Guangying(Institute of Integrated Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所

出　　处：《环球中医药》2020年第1期2-7,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(30171193);全国名老中医黄光英传承工作室

摘　　要：目的探讨补肾益气活血方对胚胎着床障碍大鼠子宫内膜孕激素受体(progesterone receptor,PR)和信号传导与活化转录因子3(signal transduction and activation of transcription factor 3,STAT3)表达的影响。方法应用米非司酮—芝麻油悬液皮下注射制造胚胎着床障碍大鼠模型,动物分为正常组、模型组、中药组和黄体酮组。中药组给予补肾益气活血方灌胃,黄体酮组给予黄体酮肌肉注射。收集妊娠第8天大鼠血清及子宫标本,观察并记录各组妊娠及胚胎着床情况,ELISA法检测血清孕酮含量,RT-PCR法检测大鼠子宫内膜PR和STAT3 mRNA的表达,免疫印迹法检测大鼠子宫内膜中PR和STAT3蛋白的表达。结果(1)与正常组相比,模型组子宫内膜发育异常且滞后,怀孕率及平均着床胚胎数均明显减少(P<0.05),孕酮明显下降(P<0.05),子宫内膜PR及STAT3蛋白表达均明显下降(P<0.05),PR mRNA表达明显下降(P<0.05),STAT3 mRNA表达无显著差异(P>0.05);(2)与模型组相比,中药组和黄体酮组子宫内膜发育明显改善,怀孕率及平均着床胚胎数均明显增加(P<0.05),孕酮水平显著增加(P<0.05),子宫内膜PR及STAT3蛋白表达均显著增加(P<0.05);中药组PR mRNA表达较模型组显著增加(P<0.05),黄体酮组PR mRNA表达较模型组无显著差异(P>0.05),两组STAT3 mRNA表达较模型组均无显著差异(P>0.05);(3)与黄体酮组相比,中药组血清孕酮含量显著降低(P<0.05),怀孕率及平均着床胚胎数、PR和STAT3的蛋白及mRNA表达均无明显差异(P>0.05)。结论补肾益气活血方能通过影响胚胎着床障碍大鼠子宫内膜PR及STAT3的表达,改善子宫内膜容受性,促进胚胎着床。Objective To investigate the effect of Bushen Yiqi Huoxue recipe on the expression of progesterone receptor(PR)and signal transduction and activation of transcription factor 3(STAT3)in endometrium of rats with embryo implantation dysfunction.Methods The rat model of embryo implantation dysfunction was made by subcutaneous injection of mifepristone and sesame oil suspension.The rats were divided into normal group,model group,traditional Chinese medicine group and progesterone group.The traditional Chinese medicine group was orally given Bushen Yiqi Huoxue recipe,and the progesterone group was injected intramuscularly with progesterone.The serum and uterus samples of rats were collected on the 8th day of pregnancy.The pregnancy rate and embryo implantation were observed and recorded,and the serum level of progesterone was detected by ELISA method.The mRNA expression of PR and STAT3 was detected by RT-PCR.The expression of PR and STAT3 in rat endometrium was detected by Western blot.Results(1)Compared with the normal group,the endometrium of the model group was dysplasia and lagged.The pregnancy rate,the average number of implanted embryos and the level of progesterone in model group were significantly lower than those in normal group(P<0.05),and the expression of PR and STAT3 in endometrium significantly decreased(P<0.05).The PR mRNA expression decreased significantly when compared to the normal group(P<0.05),while the STAT3 mRNA expression had no obvious difference(P>0.05).(2)Compared with model group,the growth of endometrium improved greatly.The pregnancy rate,average implantation embryo numbers and the level of progesterone in Chinese medicine group and progesterone group significantly increased(P<0.05),and the expression of PR and STAT3 protein in endometrium significantly increased(P<0.05).The expression of PR mRNA in Chinese medicine group were significantly higher than that in model group(P<0.05).There was no significant difference in PR mRNA expression in progesterone group compared with model group(P>0.05

关 键 词：补肾益气活血方 胚胎着床障碍 大鼠 孕激素受体 信号传导与活化转录因子3 

分 类 号：R285.5[医药卫生—中药学]