京海黄鸡白介素8基因5′调控区单核苷酸多态性及其与柔嫩艾美耳球虫抗性指标的关联性  

Correlation between Single Nucleotide Polymorphism in 5’ Regulation Region of IL-8 and Coccidiosis-Resistance of Jinghai Yellow Chicken

作　　者：王晓慧 于海亮 邹文斌 糜长浩 戴国俊[1] 张涛[1] 张跟喜[1] 谢恺舟[1] 王金玉[1] 施会强[2] WANG Xiao-hui;YU Hai-liang;ZOU Wen-bin;MI Chang-hao;DAI Guo-jun;ZHANG Tao;ZHANG Gen-xi;XIE Kai-zhou;WANG Jin-yu;SHI Hui-qiang(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009,China;Jiangsu Jinghai Poultry Group Co.,Ltd.,Haimen,Jiangsu 226100,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏京海禽业集团有限公司,江苏海门226100

出　　处：《福建农业学报》2019年第11期1262-1269,共8页Fujian Journal of Agricultural Sciences

基　　金：江苏现代农业产业技术体系建设专项(JATS[2018]303);“十二五”国家科技支撑计划项目(2014BAD13B02);江苏省高校优势学科建设工程(PAPD);国家现代农业产业技术体系建设专项(CARS-41-G23)

摘　　要：【目的】研究白介素8(IL-8)基因5′调控区单核苷酸突变对鸡柔嫩艾美耳球虫(E. tenella)抗性指标的影响。【方法】本试验利用DNA直接测序技术,检测京海黄鸡IL-8基因5′调控区单核苷酸多态性(SNPs),并对5′调控区的SNPs突变前后的转录因子进行预测,然后分析SNPs与柔嫩艾美耳球虫抗性指标的关联性。【结果】测序结果表明:IL-8基因5′调控区共检测到3个突变位点(T-550C、G-398T和T-360C),均形成了3种基因型,杂合度在0.436~0.471,PIC值在0.25~0.50,均属于中度多态,且3个突变位点均处于哈代-温伯格平衡状态。生物信息学分析表明:3个突变位点均改变了其原有的转录因子结合位点。关联分析显示:T-550C突变位点的TC型个体的IL-8表达量与TT型差异显著,TC型GSH-PX、CAT、IL-2、IL-6、IFN-γ指标均高于其他2种基因型,但差异不显著。G-398T突变位点的TT型个体SOD活性和GT型个体CAT活性均显著高于GG型;TT型个体NO含量与GG型差异极显著,与GT型差异显著;TT型IL-2表达量显著高于GT型。T-360C突变位点的TT型和TC型个体SOD活性与CC型差异极显著或差异显著;TT型和TC型个体的NO含量均显著高于CC型;TT型个体的IL-2、IL-8表达量均显著高于CC型。【结论】G-398T和T-360C突变位点的TT型个体球虫抗性优于其他基因型个体,T-550C突变位点的杂合型个体球虫抗性优于纯合型个体,表明IL-8基因5′调控区的突变对球虫抗性指标有显著的调控作用,可作为球虫病抗性鸡新品种或新品系的育种参考依据。【Objective】 Correlation between the single nucleotide polymorphism(SNPs) in the 5’ regulation region of IL-8 and the disease resistance to Eimeria tenella of chickens was studied. 【Method】 Direct DNA sequencing was performed to determine SNPs in IL-8 gene of Jinghai yellow chicken. Predicted transcription factors before and after mutation were analyzed to correlate between SNPs and coccidiosis-resistance of the birds. 【Result】 There were 3 SNPs at the mutation sites of T-550C, G-398T and T-360C detected in the target region that formed CT-, TT-and GG-genotypes with heterozygous degrees between 0.436 and 0.471 and PIC between 0.25 and 0.5. In a Hardy-Weinberg equilibrium state, SNPs were moderately polymorphic. A bioinformatics analysis confirmed that the original transcription factor binding sites in the genes had all been altered after the mutation. The expression of IL-8 of TC-genotype mutated at T-550C was significantly higher than that of TTgenotype. The coccidiosis-resistance indicators including GSH-PX, CAT, IL-2, IL-6 and IFN-γ in TC-genotype were higher than that in the other two genotypes, though not significantly. In genotypes mutated at G-398 T, the SOD activity of TTgenotype and the CAT activity of GT-genotype were significantly higher than those of GG-genotype;the NO content of TTgenotype extremely significantly different from that of GG-genotype and significantly different from that of GT-genotype;and,the IL-2 expression of TT-genotype significantly higher than that of GT-genotype. Whereas, in the genotypes mutated at T-360 C, the SOD activities of TT-and TC-genotypes were extremely significantly different or significantly different from that of CC-genotype;the NO contents of TT-and TC-genotypes significantly higher than that of the CC genotype;and, the IL-2 and IL-8 expressions of the TT genotype significantly higher than those of CC-genotypes. 【Conclusion】 It appeared that the TTgenotype with mutated G-398 T or T-360 C site would be more resistant to the parasitic attack by E. t

关 键 词：京海黄鸡 柔嫩艾美耳球虫 白介素8基因 5′调控区SNPs 抗性指标 

分 类 号：S831.2[农业科学—畜牧学]