棕榈蓟马消化道组织学与形态学研究  

Histology and Morphology of Alimentary Canal of Thrips palmi Karny

作　　者：穆野 陈粉粉[1] 魏辉 郑雪[2] 陈勇[3] 张洁[2] MU Ye;CHEN Fen-fen;WEI Hui;ZHENG Xue;CHEN Yong;ZHANG Jie(College of Life Science,Southwest Forestry University,Kunming,Yunnan 650224,China;Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China;Institute of Plant Protection,Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture,Fuzhou,Fujian 350003,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650224 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650205 [3]福建省农业科学院植物保护研究所/福建省作物有害生物监测与治理重点实验室/农业部福州作物有害生物科学观测试验站,福建福州350003

出　　处：《福建农业学报》2019年第11期1309-1314,共6页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31871936、31560499);云南省应用基础研究计划面上项目(2016FB063);云南省应用基础研究计划重点项目(2018FA020);云南省中青年学术技术带头人后备人才项目(2015HB081);福建省科技重大专项(2017NZ0003-1-4);福建省科技计划公益类专项(2017R1025-9);福建省农业科学院科技项目(STIT2017-3-2、AGY2018-5、YC2016-5)

摘　　要：【目的】棕榈蓟马Thrips palmi Karny不仅可以直接取食为害作物,而且能够传播正番茄斑萎病毒属Orthotospovirus病毒病害,给我国蔬菜、花卉等经济作物造成了严重危害。本研究探讨了棕榈蓟马成虫消化道的组织学和形态学结构,旨在为今后研究正番茄斑萎病毒属病毒在棕榈蓟马体内的侵染路径提供依据。【方法】利用激光共聚焦显微镜(confocal laser scanning microscopy, CLSM)观察棕榈蓟马消化道整体形态结构,利用透射电子显微镜(transmission electron microscope,TEM)观察棕榈蓟马消化道超微结构。【结果】棕榈蓟马消化道由前肠、中肠、后肠和马氏管组成。前肠包括咽、食道、嗉囊和贲门。中肠分为前中肠、中中肠和后中肠3个部分;中肠没有围食膜,前中肠微绒毛表面有微绒毛膜,后中肠肠壁根据细胞形态分为褶皱端和扁平端,微绒毛没有微绒毛膜。后肠由回肠和直肠组成;回肠的肠壁肌肉发达,肠腔周围有一层角质层;直肠内部有大量微生物。马氏管有4根,着生在中肠与后肠的分界线处;马氏管处微绒毛密集但形状不规则,管壁细胞内有大量囊泡。【结论】本研究描述了棕榈蓟马消化道的形态与组成,分析了不同部位的功能,为研究病原物在蓟马体内的侵染路径奠定基础。【Objective】 Histology and morphology of the alimentary canal of Thrips palmi Karny(Thysanoptera: Thripidae),a pest that causes serious damage to its host crops by not only feeding on them but also transmitting viruses, were studied to investigate the infection route of Orthotospovirus on plants. 【Method】 Structure of the alimentary canal in T. palmi was examined under the laser scanning confocal microscopy, and the transmission electron microscopy applied for the ultrastructure observation. 【Result】 The alimentary canal was shown to consist of foregut, midgut, hindgut, and malpighian tubules. The foregut included pharynx, oesophagus, crop, and cardia. The midgut was made of anterior, middle and posterior parts with no peritrophic membrane. In the anterior midgut, a thin membrane was present on the surface of microvilli. Morphologically, the intestinal wall of posterior midgut had a pleated and a flat side with no membrane on the microvilli surface. The hindgut composed of ileum and rectum. The ileum had strong muscles and a cuticle layer covering the lumen. Inside the rectum,microorganisms seemed to thrive. Four malpighian tubules were located at the junction between the midgut and hindgut extending freely into the hemocoel with numerous vesicles in the cells and abundant microvilli around the irregular-shaped lumen. 【Conclusion】 The morphology and functions of the alimentary canal in T. palmi were closely examined to initiate further studies on the pathogenic infection route on crops through the insect.

关 键 词：缨翅目 蓟马科 棕榈蓟马 消化道 形态学 

分 类 号：S435[农业科学—农业昆虫与害虫防治]