血管紧张素原基因单核苷酸多态性与心房颤动的相关性及临床价值研究  

Association between single nucleotide polymorphism of angiotensinogen gene and atrial fibrillation and its clinical value

作　　者：向丽 张维贞 黄山[2] 田野[3] XIANG Li;ZHANG Weizhen;HUANG Shan;TIAN Ye(Clinical Laboratory Medicine College,Guizhou Medical University,Guiyang,Guizhou 550002,China;Clinical Laboratory Center,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 550002,China;Department of Cardiology,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 550002,China)

机构地区：[1]贵州医科大学检验学院,贵州贵阳550002 [2]贵州省人民医院临床检验中心,贵州贵阳550002 [3]贵州省人民医院心内科,贵州贵阳550002

出　　处：《检验医学与临床》2020年第3期301-304,308,共5页Laboratory Medicine and Clinic

基　　金：贵州省科技厅社发公关项目[黔科合SY(2015)3045号]

摘　　要：目的探讨血管紧张素原(AGT)基因rs5046、rs2148582单核苷酸多态性(SNP)与心房颤动(房颤)的相关性,为临床对房颤的早期诊断及治疗提供参考依据。方法以100例健康体检者为房颤组,108例房颤患者为对照组,并提取两组全血基因组,对血管紧张素原基因rs5046、rs2148582多态性位点进行序列扩增,继而对AGT基因这两个位点的扩增序列进行一代测序分析;并对AGT基因的rs5046、rs2148582位点多态性与房颤的患病风险进行关联分析。结果房颤组rs5046位点的CT、TT基因型频率明显高于对照组(P<0.05),TT基因型对房颤的影响大于CT基因型,C和T等位基因频率比较,差异有统计学意义(P<0.05)。结论 AGT基因rs5046多态性位点与房颤易感性相关,其等位基因T可增加房颤患病的风险。Objective To investigate the relationship between single nucleotide polymorphism of angiotensin-progenitor rs5046 and rs2148582 and the genetic susceptibility of atrial fibrillation,so as to provide for the early diagnosis and treatment of atrial fibrillation.Methods A total of 100 healthy physical examination people were taken as the control group,and 108 patients with atrial fibrillation were taken as the case group.Then the blood cells of the control group and the case group were collected,then their blood genome were extracted.The polymorphism sites of AGT gene rs5046 and rs2148582 were amplified,and the gene sequences of the two sites of AGT gene were detected by first-generation sequencing technology.The chi-square test was used to analyze the association between the polymorphism of AGT rs5046 with rs2148582 and the risk for atrial fibrillation.Results The frequencies of CT and TT genotypes at rs5046 locus in the atrial fibrillation group were significantly higher than that of the control group(P<0.05);the influence of TT genotype on atrial fibrillation was greater than that of CT genotype,and the allele frequency between C and T was significantly different between the two groups(P<0.05).Conclusion The polymorphism of AGT rs5046 is associated with atrial fibrillation susceptibility,and the increased risk of atrial fibrillation is associated with the allele T.

关 键 词：心房颤动 血管紧张素原基因 单核苷酸多态性 诊断价值 

分 类 号：R541[医药卫生—心血管疾病]