麻黄-甘草药对抑制过敏性哮喘的效应及机制初探  

Effects and Mechanism of Mahuang-Gancao Couplet Medicines on Allergic Asthma

作　　者：袁为远 魏盼 包凯帆 姚露 王霄彤 王思齐 郑劼[4] 洪敏[1,2] 江国荣 YUAN Wei-yuan;WEI Pan;BAO Kai-fan;YAO Lu;WANG Xiao-tong;WANG Si-qi;ZHENG Jie-;HONG Min;JIANG Guo-rong(School of Pharmacy,Nanjing University of ChinesE-Medicine,Nanjing,210023,China;Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of ChinesE-Materia Medica,Nanjing,210023,China;ThE-Affiliated Suzhou Hospital of Traditional ChinesE-MedicinE-of Nanjing University of ChinesE-Medicine,Suzhou,215003,China;School of MedicinE-&Holistic IntegrativE-Medicine,Nanjing University of ChinesE-Medicine,Nanjing,210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药药效与安全评价重点实验室,江苏南京210023 [3]南京中医药大学附属苏州市中医医院,江苏苏州215003 [4]南京中医药大学医学院·整合医学学院,江苏南京210023

出　　处：《南京中医药大学学报》2020年第1期41-45,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81703733,81473395);江苏省中药药效与安全性评价重点实验室开放课题(JKLPSE201603)

摘　　要：目的研究麻黄-甘草药对对过敏性哮喘的影响,并初步探讨其作用机制。方法建立屋尘螨(HDM)诱导的过敏性哮喘模型,造模同时给予麻黄-甘草药对提取物(MG)。使用无创肺功能仪检测小鼠气道反应性,HE染色观察小鼠肺组织病理学变化,Masson染色观察小鼠肺组织的胶原沉积情况,进行血液嗜酸性粒细胞(EOS)计数,ELISA法检测支气管肺泡灌洗液(BALF)及肺组织IL-4、IL-5、IL-13、TGF-β1及血清IgE的表达,免疫组化检测肺组织E-cadherin、N-cadherin、Vimentin、α-SMA和TGF-β1蛋白表达情况。结果 MG可显著降低气道高反应性,抑制BALF及肺组织IL-4、IL-5和IL-13的表达,降低血液EOS及血清IgE的表达,并且减轻肺组织的炎症浸润及胶原沉积。进一步结果表明MG可抑制肺组织TGF-β1的表达,上调E-cadherin以及下调N-cadherin、Vimentin、α-SMA的表达。结论 MG能够通过抑制支气管上皮间质转化,从而缓解气道的炎症状态以及改善哮喘的气道重塑,进而发挥对过敏性哮喘的治疗作用。OBJECTIVE To investigate the effect and mechanism of Mahuang-Gancao couplet medicines(MG)on allergic asthma.METHODS The airway responsiveness was detected by non-invasive pulmonary function test.The pathological changes of lung tissue were observed by HE staining.The collagen deposition of lung tissue was observed by Masson staining.The eosinophil(EOS)count in blood was performed.IL-4,IL-5,IL-13,TGF-β1 and IgE were detected by ELISA assay.The expression of E-cadherin,N-cadherin,Vimentin,α-SMA and TGF-β1 were detected by immunohistochemistry.RESULTS MG significantly reduced airway hyperresponsiveness,inhibited the expression of IL-4,IL-5 and IL-13 in BALF and lung tissue,decreased the expression of serum IgE,and alleviated inflammation of lung tissue.MG inhibited the expression of TGF-β1 in lung tissue,increased the expression of E-cadherin and down-regulated the expression of N-cadherin,Vimentin and α-SMA.CONCLUSION MG can play a key role in the treatment of allergic asthma by inhibiting the bronchial epithelial-mesenchymal transition,thereby alleviating the inflammatory state of the airway and improving the airway remodeling of asthma.

关 键 词：麻黄-甘草药对 上皮间质转化 气道重塑 过敏性哮喘 

分 类 号：R285.5[医药卫生—中药学]