糖肾宁对高糖诱导的足细胞损伤模型Rho/ROCK通路的影响  

Effects of Tangshenning on High Glucose-Induced Podocyte Injury Based on Rho/ROCK Pathway

作　　者：徐嘉一 高彦彬[1,2] 邹大威 王晓磊[1,2] 王涛 师一民[1,2] 吴冰杰 Xu Jiayi;Gao Yanbin;Zou Dawei;Wang Xiaolei;Wang Tao;Shi Yimin;Wu Bingjie(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Beijing Key Laboratory of TCM Collateral Disease Theory Research,Beijing 100069,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中医络病研究北京市重点实验室,北京100069

出　　处：《世界中医药》2019年第12期3164-3168,共5页World Chinese Medicine

基　　金：北京市自然科学基金项目(7172032);国家自然科学青年基金项目(81302951);科技部国家重点研发计划(2018YFC1704100)

摘　　要：目的:基于Rho/ROCK信号通路研究中药糖肾宁对高糖诱导足细胞损伤模型的影响。方法:高糖培养足细胞,以不同药物分组干预足细胞24 h,用罗丹明染色的鬼笔环肽法检测足细胞骨架,细胞免疫荧光检测足细胞标志蛋白Nephrin,Western Blotting检测RhoA、ROCK1、Nephrin蛋白的表达。比较不同药物或药物浓度对高糖诱导足细胞损伤模型的影响。结果:糖肾宁可改善足细胞骨架结构,下调高糖诱导的足细胞损伤模型中异常升高的RhoA和ROCK1蛋白表达,上调Nephrin表达。结论:中药糖肾宁可抑制高糖诱导的足细胞损伤模型RhoA/ROCK1信号通路,减轻足细胞骨架重构,改善足细胞损伤。Objective:To investigate the mechanism of traditional Chinese medicine Tangshenning on high glucose-induced podocyte injury based on Rho/ROCK signal pathway.Methods:Podocytes were cultured in high glucose.The podocytes were treated with different drugs for 24 h.The podocyte cytoskeleton was detected by rhodamine-stained phalloidin.The podocyte marker protein Nephrin was detected by immunofluorescence.Western blotting was used to detect RhoA,ROCK1 and Nephrin protein.Results:Tangshenning could improve the podocyte cytoskeleton,decreased expression of RhoA and ROCK1 protein,and increased expression of high glucose-induced Nephrin expression in high glucose-induced podocyte injury model.Conclusion:Traditional Chinese medicine Tangshenning can reduce the expression of RhoA/ROCK1 signaling pathway-related protein in high glucose cultured podocytes,reduce podocyte cytoskeletal remodeling,increase the expression of high glucose-induced Nephrin protein,and improve high glucose-induced podocyte injury.

关 键 词：中药 糖肾宁 高糖 足细胞损伤 Rho/ROCK通路 实验研究 足细胞标志蛋白 糖尿病肾病 

分 类 号：R242[医药卫生—中医临床基础] R587.1[医药卫生—中医学]