脑心通对高血压大鼠左心室重构的影响及机制  

Effects and Mechanism of Naoxintong on Left Ventricular Remodeling in Hypertensive Rats

作　　者：王景武 李彬彬 孙克陆 吴永祥 Wang Jingwu;Li Binbin;Sun Kelu;Wu Yongxiang(First Department of Cardiology,901 Hospital of the Chinese People′s Liberation Army,Hefei 230031,China;Emergency Room,Jiangsu People′s Hospital,Nanjing 210000,China)

机构地区：[1]解放军第901医院心内一科,合肥230031 [2]江苏省人民医院急诊室,南京210000

出　　处：《世界中医药》2019年第12期3198-3201,共4页World Chinese Medicine

基　　金：江苏省科技厅社会发展项目(BL2014088)

摘　　要：目的:探讨脑心通对自发性高血压(SHR)大鼠左心室重构的影响及机制。方法:将SHR大鼠随机分为低/高剂量组、模型组和对照组,每组20只,15只WKY大鼠为正常组。模型组灌胃30.0 mg/kg卡托普利,低/高剂量组灌胃1.0、2.0 g/kg脑心通,正常组、模型组大鼠则灌胃等量生理盐水,1次/d,各组均干预3个月。分别于干预4、8、12周检测各组大鼠尾动脉收缩压,超声心动图评估各组大鼠左心室形态及左心收缩功能,用苏木精-伊红(HE)染色观察大鼠心肌组织病理学形态,蛋白免疫印迹法检测各组大鼠心肌组织中PPARα、PPARγ蛋白表达。结果:与正常组比较,术后4周、8周、12周模型组尾动脉收缩压升高;而低/高剂量组和对照组较模型组降低,且高剂量组低于低剂量组,差异有统计学意义(P<0.05)。模型组LVDd、LVDs较正常组升高,而低/高剂量观察组和对照组较模型组降低,差异有统计学意义(P<0.05);模型组LVSd、LVSs、LVEF、FS较正常组降低,而低/高剂量组和对照组较模型组升高,差异有统计学意义(P<0.05)。与正常组比较,模型组大鼠心肌组织中PPARα、PPARγ蛋白水平升高,差异有统计学意义(P<0.05),而对照组及低/高剂量组较模型组降低,差异有统计学意义(P<0.05)。结论:脑心通可剂量依赖性降低SHR大鼠血压,减轻大鼠左心室重构,其机制可能与上调PPARα、PPARγ蛋白表达相关。Objective:To investigate the effects and mechanism of Naoxintong on left ventricular remodeling in spontaneous hypertension(SHR)rats.Methods:SHR rats were randomly divided into a low/high dose experimental group,a model group and a control group,with 20 rats in each group and 15 WKY rats in the normal group.The model group was given 30.0 mg/kg captopril,and the low/high dose experimental group was given 1.0 and 2.0 g/kg Naoxintong,and the model group and the normal group were given the same amount of saline once a day.All rats were intervened for 3 months.Systolic blood pressure of tail artery was measured at 4,8 and 12 weeks after intervention.Left ventricular morphology and left ventricular systolic function were evaluated by echocardiography.Myocardial histopathological morphology was observed by hematoxylin-eosin(HE)staining.PPARαand PPARγprotein in myocardium were detected by Western blotting(WB).Results:Compared with the normal group,the systolic pressure of tail artery in the model group increased at 4,8 and 12 weeks after operation(P<0.05);compared with the model group,the systolic pressure of tail artery in the low/high dose experimental group and the control group decreased at 4,8 and 12 weeks after intervention,and the systolic pressure of tail artery in the high dose experimental group was lower than that in the low dose experimental group(P<0.05).LVDd and LVDs in model group were higher than those in normal group,while those in low/high dose group and control group were lower than those in model group(P<0.05);LVSd,LVSs,LVEF and FS in the model group were lower than those in normal group,while those in the low/high dose group and the control group were higher than those in the model group(P<0.05).Compared with the normal group,the contents of PPARαand PPARγprotein in the myocardium of the model group increased(P<0.05).Compared with the model group,the contents of PPARαand PPARγprotein in the myocardium of the control group and the low/high dose experimental group decreased significantly(P<0.05).

关 键 词：高血压 脑心通 血压 心肌损伤 过氧化物酶体增殖物激活受体 

分 类 号：R283[医药卫生—中药学] R544[医药卫生—中医学]