CD4^+细胞与CD8^+细胞比值与新生儿缺氧缺血性脑病的相关性分析  

Analysis on correlation between the ratio of CD4^+ cells to CD8^+ cells and neonatal hypoxic-ischemic encephalopathy

作　　者：黎巧茹 邹亚伟[2] 赖文英[1] 林春燕[1] 李丽羽 LI Qiaoru;ZOU Yawei;LAI Wenying;LIN Chunyan;LI Liyu(Department of Pediatrics,Zhongshan People’s Hospital,Guangdong,Zhongshan 528400,China;Department of Pediatrics,the First Affiliate Hospital of Guangzhou Medical University,Guangdong,Guangzhou 510000,China)

机构地区：[1]广东省中山市人民医院普通儿科,广东中山528400 [2]广州医科大学附属第一医院儿科,广东广州510000

出　　处：《中国医药科学》2020年第1期14-16,58,共4页China Medicine And Pharmacy

基　　金：广东省自然科学基金（2016A030313654）

摘　　要：目的明确CD4^+细胞与CD8^+细胞比值与新生儿缺氧缺血性脑病病情的相关性。方法选取2015年10月~2018年6月中山市人民医院新生儿科住院的60例HIE患儿及同期我院产科出生正常新生儿73例。应用流式细胞仪测定血CD3^+、CD4^+、CD8^+细胞及CD4^+/CD8^+比值。结果HIE患儿T细胞亚群的表达均明显低于正常新生儿(P均<0.05);重度HIE患儿CD3^+、CD4^+细胞及CD4^+/CD8^+比值明显均低于中度HIE患儿(P均<0.05),中度HIE患儿CD3^+、CD4^+细胞及CD4^+/CD8^+比值明显均低于轻度HIE患儿(P均<0.05),而不同分度HIE的CD8^+细胞则无统计学差异(P>0.05);CD3^+、CD4^+及CD4^+/CD8^+与HIE病情分度均呈负相关(P均<0.05)。结论CD4^+/CD8^+比值与HIE新生儿的病情危重程度密切相关,可作为患儿病情严重程度的有效预测指标。Objective To determine the correlation between the ratio of CD4^+cells to CD8^+cells and neonatal hypoxic-ischemic encephalopathy.Methods 60 neonates with HIE who were hospitalized in department of neonatology in Zhongshan People’s Hospital and 73 normal neonates who were born in department of obstetrics in our hospital from October 2015 to June 2018 were selected.CD3^+,CD4^+,CD8^+ cells and the ratio of CD4^+/CD8^+ were measured by flow cytometry.Results The expression of T lymphocyte subsets in patient with HIE was significantly lower than that in normal newborns(P<0.05).The levels of CD3^+ and CD4^+ and CD4^+/CD8^+ ratio in patient with severe HIE were significantly lower than those in patient with moderate HIE(P<0.05).The levels of CD3^+,CD4^+cells and CD4^+/CD8^+ ratio in patient with moderate HIE was significantly lower than those in patient with mild HIE(P<0.05).But the CD8^+ cells in different grades of HIE had no statistically significant difference(P>0.05).CD3^+,CD4^+ and CD4^+/CD8^+ were negatively correlated with the severity of HIE(P<0.05).Conclusion The ratio of CD4^+/CD8^+ is closely related to the severity of neonates with HIE,which can be used as an effective predictor of the severity of neonates.

关 键 词：新生儿缺氧缺血性脑病 淋巴细胞 T淋巴细胞亚群 CD4+T淋巴细胞 CD8+T淋巴细胞 

分 类 号：R722.1[医药卫生—儿科]