基于大生产考察烫制对骨碎补中柚皮苷含量的影响  

Influence of Stir-Baked with Sand Methods on Naringin Content in Rhizoma Drynariae Based on Mass Production

作　　者：马杰 丁野 罗艳 李瑞莲 王湘波 MA Jie;DING Ye;LUO Yan;LI Ruilian;WANG Xiangbo(Hunan Institute for Drug Control,Changsha,Hunan,China 410001;Hunan Engineering&Technology Research Center for Pharmaceutical Quality Evaluation,Changsha,Hunan,China 410001)

机构地区：[1]湖南省药品检验研究院,湖南长沙410001 [2]湖南省药品质量评价工程技术中心,湖南长沙410001

出　　处：《中国药业》2020年第3期38-41,共4页China Pharmaceuticals

基　　金：《中国药典》药品标准提高课题[420]

摘　　要：目的考察烫制对骨碎补中柚皮苷含量的影响及其影响因素。方法建立烫骨碎补中柚皮苷含量的高效液相色谱法。实地参与中药饮片生产企业烫骨碎补的砂烫炮制过程,考察不同温度、炮制时长对柚皮苷含量的影响,并测定不同企业的多批次骨碎补药材及相对应的烫骨碎补饮片中柚皮苷的含量。结果柚皮苷进样量线性范围为3. 561 2~2 226 ng(r=0. 999 9),精密度、稳定性、重复性试验的RSD均小于3. 0%,平均加样回收率为99. 58%,RSD为1. 31%(n=9)。随着炮制时间的延长,柚皮苷含量逐渐降低,且受烫制温度影响。收集的10批次烫骨碎补与烫制前相比,柚皮苷含量4批次明显升高,2批次明显降低,4批次未见明显变化。结论建立含量测定的方法简便、准确,可用于测定烫骨碎补柚皮苷的含量。烫制时温度、时长及原料规格均一性是影响柚皮苷含量的关键因素。Objective To investigate the influence of stir-baked with sand methods on naringin content in Rhizoma Drynariae and its influencing factors. Methods The determination method of naringin content in Rhizoma Drynariae preparations stir-baked with sand was established by high performance liquid chromatography( HPLC). We participated in the processing of Rhizoma Drynariae in Chinese herbal decoction pieces enterprises,investigated the influences of different temperatures and processing time on the naringin content,and determined the content of naringin in many batches of Rhizoma Drynariae and their processed sample products from different enterprises. Results The linear range of naringin was 3. 561 2-2 226 ng( r = 0. 999 9). The RSDs of precision,stability and repeatability tests were all less than 3. 0%. The average recovery of adding samples was 99. 58%,RSD was 1. 31%( n = 9). The content of naringin decreased gradually with the increase of processing time,and the temperature of stir-bake also affected the content. Among the10 batches of Rhizoma Drynariae after stir-bake with sand,the content of naringin in 4 batches were obviously decreased,2 batches were significant increased,and 4 batches had no changes. Conclusion The content determination method is simple and accurate,which can be used to determine the content of naringin in Rhizoma Drynariae preparations stir-baked with sand. The temperature,time and homogeneity of raw material specifications during stir-bake with sand are the key influencing factors for the naringin content.

关 键 词：大生产 骨碎补 砂烫法 柚皮苷 影响因素 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]