油用牡丹凤丹种子休眠解除及组织培养研究  

Study on the Release of Dormancy and Regeneration of Paeonia ostii Variety ’Fengdan’

作　　者：韦祖粉 蒲振蕊 鲁清清 王娟 蓝增全 吴田[1] WEI Zufen;PU Zhenrui;LU Qingqing;WANG Juan;LAN Zengquan;WU Tian(Southwest Landscape Architecture Engineering Research Center of State Forestry andGrassland Administration/University College of Landscape Architecture and Horticulture Sciences Southwest Forestry,Kunming 650224,China;College of Life Sciences,Kunming 650224,China;Southwest Institute of Green Development,Kunming 650224,China)

机构地区：[1]国家林业草原局西南风景园林工程技术研究中心/西南林业大学园林园艺学院,云南昆明650224 [2]西南林业大学生命科学学院,云南昆明650224 [3]西南林业大学绿色发展研究院,云南昆明650224

出　　处：《种子》2020年第1期54-58,63,共6页Seed

基　　金：云南省科技计划(对外科技合作计划——国际科技合作)——滇牡丹重要经济性状研究与综合利用技术引进(2015 IA 005);云岭产业技术领军人才项目资助(云发改人事[2018]212号)

摘　　要：以油用牡丹凤丹种子为材料,分析微波辐射及赤霉素浸泡处理对其休眠解除的影响,并在此基础上探讨在组培离体再生中不同激素配比对解除休眠处理种子的胚萌发、丛生芽诱导以及生根率的影响。结果表明:在1000 mg·L^-1赤霉素(GA 3)浸泡24 h后,凤丹种子的萌发效果最佳培养基为WPM+1 mg·L^-1 PVP+5 mg·L^-1 GA 3+1.5 mg·L^-16-BA+5 mg·L^-1 Ca(NO 3)2;丛生芽诱导增殖培养基为WPM+1.5 mg·L^-1 IBA+2 mg·L^-16-BA+2 mg·L^-1 PVP+250 mg·L^-1 Ac,增殖系数为2.20;诱导丛生芽生根培养基为WPM+2 mg·L^-1 IBA+1.5 mg·L^-1 NAA,生根率为55.56%。The effects of microwave radiation and gibberellin immersion treatment on the release of dormancy of Fengdan seeds were analyzed,and on this basis,the effects of different hormone ratios in tissue culture on embryo germination,shoot induction and rooting rate of dormancy treated seeds were also discussed.The results showed that the optimal medium for the seed germination was WPM+1 mg·L^-1 PVP+5 mg·L^-1 GA 3+1.5 mg·L^-16-BA+5 mg·L^-1 Ca(NO 3)2 after soaking 1000 mg·L^-1 gibberellin(GA 3)for 24 h,and the growth medium for tufted bud induction was WPM+1.5 mg·L^-1 IBA+2 mg·L^-16-BA+2 mg·L^-1 PVP+250 mg·L^-1 Ac,with the multiplication coefficient being 2.20.Finally,the rooting induction medium for clustered buds was WPM+2 mg·L^-1 IBA+1.5 mg·L^-1 NAA,with the rooting rate being 55.56%.

关 键 词：油用牡丹 离体再生 丛生芽 生根 

分 类 号：S685.11[农业科学—观赏园艺]