鸭茅成熟种子愈伤组织诱导影响因素  

Study of factors affecting callus induction from mature seeds of orchardgrass

作　　者：许蕾 韩佳婷 冯光燕 刘亚男 黄琳凯[1] 张新全[1] XU Lei;HAN Jiating;FENG Guangyan;LIU Ya’nan;HUANG Linkai;ZHANG Xinquan(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]四川农业大学动物科技学院,四川成都611130 [2]中国农业科学院畜牧兽医所,北京100081

出　　处：《草业科学》2020年第1期87-97,共11页Pratacultural Science

基　　金：国家自然科学基金(NSFC 31872997);国家现代牧草产业技术体系(CARS-34)

摘　　要：以鸭茅(Dactylis glomerata)成熟种胚为外植体,探讨了种子预处理、品种选择、培养基及激素配比对其愈伤组织诱导过程的影响,为鸭茅高频再生体系建立奠定了基础。结果表明,3个预处理条件中,次氯酸钠浓度对安巴鸭茅种子愈伤诱导率影响达显著水平(P<0.05)。10个鸭茅品种中,以安巴愈伤诱导能力最佳。不同培养基愈伤诱导率及愈伤形成后的形态发育存在差异,SH、MS、LS培养基均具有较高的愈伤诱导率,其中SH诱导效果最好,愈伤呈现淡黄或黄、干爽松脆。在添加2,4-D的基础上配合30μmol·L^–1 dicamba可以促进鸭茅种子的愈伤诱导;同时,2,4-D与较低浓度的6-KT组合也有利于愈伤的诱导。综上所述,鸭茅种子先放置于4℃处理24 h,而后用75%酒精处理5 min,1.1%NaClO灭菌处理30 min,最佳诱导培养基为SH+2.0 mg·L^–1 CPA+0.1 mg·L^–16-KT+30μmol·L^–1dicamba+0.5 mg·L^–12,4-D,愈伤诱导效率较高且在后期较易形成胚性愈伤,初步建立了一套鸭茅高效稳定的组织培养再生体系。To establish an efficient regeneration system for orchardgrass,mature seeds were studied to determine the effects of seed pretreatment,variety selection,medium selection,and hormone ratio on the process of callus induction.The results showed a statistically significant(P<0.05)effect of NaClO concentration on callus induction rate among the three pretreatment conditions.The cultivar‘Anba’had the strongest callus induction ability among the ten cultivars.Callus induction ability and morphological development were related to the medium used:Schenk&Hildebrandt medium(SH),Murashige&Skoog medium(MS),and Linsmaier&Skoog medium(LS)(all resulted in higher callus induction rates;of these,SH had the best induction effect and yellowish or yellow,dry,and crisp calli were obtained).It was thought that 2,4-D with 30μmol·L^–1 dicamba would promote callus induction;in addition,the combination of 2,4-D and a lower concentration 6-KT was also beneficial for callus induction.In conclusion,the seeds of‘Anba’were first placed at 4℃ for 24 h after soaking,then treated with 75%alcohol for 5 min,and sterilized with 1.1%NaClO for 30 min.The optimum medium for callus induction was SH+2.0 mg·L^–1 CPA+0.1 mg·L^–16-KT+30μmol·L^–1 dicamba+0.5 mg·L^–12,4-D.With this medium and hormone combination,the callus induction efficiency was high and embryonic calli were more readily induced.Thus,an efficient and stable preliminary tissue culture and regeneration system was established for orchardgrass.

关 键 词：鸭茅 成熟种子 愈伤诱导 预处理 2 4-D DICAMBA 再生 

分 类 号：S543.3[农业科学—作物学]