黄芩提取物下调Piwil2增强他莫昔芬对乳腺癌细胞敏感性的分析  

Scutellaria extract enhances the sensitivity of tamoxifen to breast cancer cells by down-regulating piwil2

作　　者：刘欢[1] 陈晓燕[1] 刘燕玲[1] LIU Huan;CHEN Xiao-yan;LIU Yan-ling(Jiangxi University of Technology,Nanchang Jiangxi 330000,China)

机构地区：[1]江西科技学院

出　　处：《时珍国医国药》2019年第11期2591-2593,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81673009);江西省教育厅科学技术基金(GJJ180971);江西省教育科学“十三五”规划基金(19YB243);江西省卫生计生委科技计划基金(20193014)

摘　　要：目的探讨黄芩提取物能否通过调节Piwil2的表达来增强他莫替芬对乳腺癌MDA-MB-231、MCF-7细胞的敏感性。方法通过MTT检测他莫昔芬以及黄芩提取物与他莫昔芬联合作用于MDA-MB-231、MCF-7细胞后,24~96h各组细胞的增殖抑制率;通过RT-PCR检测黄芩提取物、他莫昔芬、黄芩提取物与他莫昔芬联合作用MDA-MB-231、MCF-7细胞72h,各组细胞Piwil2 mRNA的表达情况。结果他莫昔芬作用于MDA-MB-231、MCF-7细胞24~96h后增殖抑制率显著低于黄芩提取物与他莫昔芬联合作用MDA-MB-231、MCF-7细胞。黄芩提取物与他莫昔芬联合作用MDA-MB-231细胞72h,细胞Piwil2 mRNA的表达水平明显低于他莫昔芬组,与黄芩提取物组比较差异不明显。结论黄芩提取物可能通过下调Piwil2的表达来增强他莫替芬对乳腺癌细胞的敏感性。Objective To investigate whether Scutellariae Radix extract can enhance the sensitivity of tamoxifen to breast cancer MDA-MB-231, MCF-7 cells by regulating the expression of Piwil2. Methods MTT assay was used to detect the proliferation inhibition rate of tamoxifen and Scutellaria extract combined with tamoxifen in MDA-MB-231 and MCF-7 cells from 24 h to 96 h;the expression of Piwil2 mRNA in MDA-MB-231 and MCF-7 cells was detected by reverse transcription-polymerase chain reaction(RT-PCR) for 72 hours after treatment with skullcap extract, tamoxifen and skullcap extract.Results The inhibition rate of tamoxifen on MDA-MB-231 and MCF-7 cells was significantly lower than that of Astragalus extract and tamoxifen in MDA-MB-231 and MCF-7 cells after 24 to 96 hours. Scutellaria extract combined with tamoxifen MDA-MB-231 and MCF-7 cells 72 h, the expression level of Piwil2 mRNA was significantly lower than the tamoxifen group, compared with the skullcap extract group was no significant difference. Conclusion Scutellaria extract may enhance the sensitivity of tamoxifen to breast cancer cells by down-regulating piwil2 expression.

关 键 词：黄芩提取物 Piwil2 他莫替芬 乳腺癌 

分 类 号：R979[医药卫生—药品]