β-细辛醚对多房棘球蚴的体外杀伤作用  

Study on the β-Asarone against Echinococcus Multilocularis protoscoleces in vitro

作　　者：郑佳 李占强[1,2] 杨全余[1,2] 廖梦姣[4] 张颖 胡缤文 汤锋 李润乐[2] ZHENG jia;LI Zhanqiang;YANG Quanyu;LIAO Mengjiao;ZHANG Ying;HU Bingwen;TANG Feng;LI Runle(Qinghai University Research Center for High altitude Medicine,Key Laboratory of Application and Foundation for High Altitude Medicine Research of Qinghai Province,Key Laboratory of Ministry of Education for High Altitude Medicine,Qinghai University,Xining,Qinghai,810001;Qinghai University Medical college,Xining,Qinghai,810001;Qinghai Provincial People's Hospital,Xining 810001;The fifth Affiliated Hospital of Zunyi Medical University,Zunyi,Guizhou,536000)

机构地区：[1]青海大学高原医学研究中心,青海省高原医学应用基础重点实验室,高原医学教育部重点实验室,青海西宁810001 [2]青海大学医学院,青海西宁810001 [3]青海省人民医院,青海西宁810001 [4]遵义第五附属医院,贵州遵义536000

出　　处：《中国高原医学与生物学杂志》2019年第4期256-259,共4页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省科技厅应用与基础研究项目(2017-ZJ-703);国家自然科学基金项目(81860299);青海省农牧厅科技项目(NMSY-2017-09)

摘　　要：目的明确α-细辛醚和β-细辛醚在体外对多房棘球蚴的杀伤作用。方法将5 mg/mL的α-细辛醚和β-细辛醚在体外分别与多房棘球蚴共培养时用光镜观察虫体变化,并以不同浓度的β-细辛醚在体外与多房棘球蚴共培养,以伊红染色后观察虫体的死亡率及结构变化。结果将多房棘球蚴分别与α-细辛醚、β-细辛醚在体外作用0.5 h,后者全部死亡,前者无变化。将多房棘球蚴在体外分别与2.50、1.25 mg/mL的β-细辛醚作用,前者在2 h时全部死亡,后者在20 h时死亡;浓度为0.75 mg/mL时,在20 h后多房棘球蚴死亡率达64.33%±2.333;扫描电镜观察发现多房棘球蚴虫体表面损伤,头部微绒毛消失、钩疑似脱落。结论β-细辛醚在体外对多房棘球蚴具有较强的杀伤作用。Objective To identify the effects ofα-asarone andβ-asarone against protoscoleces in vitro.Methods The protoscoleces were treated with 5 mg/mLβ-asarone andα-asarone in vitro and observed the mortality.Used different concentrations ofβ-asarone in vitro and observed the mortality and structural changes of the protoscoleces under light microscope and SEM.Result The Echinococcus multilocularis protoscoleces was cultured withβ-asarone andα-asarone respectively,after 0.5 h,β-asarone group was all died andα-asarone group was alive.When incubated with 2.50 and 1.25 mg/mLβ-asarone,after 2 hours and 20 hours protoscoleces were all died,respectively.The mortality of protoscoleces was 64.33±2.333%when incubated with 0.75 mg/mLβ-asarone for 20 hours.The results of SEM showed that the surface of Echinococcus multilocularis protoscoleces were damaged,microvilli were disappeared,and the hooks were fell off after treated with 2.5 mg/mLβ-asarone.Conclusionβ-asarone has a stronger killing effect on Echinococcus multilocularis protoscoleces in vitro.

关 键 词：Β-细辛醚 Α-细辛醚 多房棘球蚴 药理 作用 

分 类 号：R917[医药卫生—药物分析学]