同型半胱氨酸对雌激素受体α基因甲基化调控及其与原发性高血压相关性分析  

Role of Hcy in regulating methylation of estrogen receptorαgene promoter and its relationship with essential hypertension

作　　者：刘东芳 杨志甫[1] 刘丹[1] 王丽珍[4] 孙怀玉[2] 乔庆红[3] Liu Dongfang;Yang Zhifu;Liu Dan;Wang Lizhen;Sun Huaiyu;Qiao Qinghong(First Affiliated Hospital of Inner Mongolia University of Science and Technology Baotou Medical School,Baotou 014010,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院神经内三病区,014010 [2]内蒙古科技大学包头医学院第一附属医院病房超声科,014010 [3]内蒙古科技大学包头医学院第一附属医院心内科,014010 [4]内蒙古科技大学包头医学院基础与法医学院病理教研室

出　　处：《中华老年心脑血管病杂志》2020年第1期20-23,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：内蒙古自治区高等学校科学研究项目（NJZY16210）;包头市科技计划项目（2015S2004-5-7）

摘　　要：目的观察原发性高血压(EH)患者雌激素受体α(ER-α)基因启动子区域甲基化状态,分析其与血浆同型半胱氨酸(Hcy)水平和颈动脉内膜中层厚度(CIMT)的相关性。方法选择EH患者70例为EH组,其中高血压1级24例、高血压2级23例、高血压3级23例。选同期健康体检者50例为对照组。用直接化学发光法检测血浆Hcy水平,Hcy正常47例(Hcy<15μmol/L)和高同型半胱氨酸血症(HHcy,Hcy≥15μmol/L)23例。用巢式甲基化特异性PCR法检测ER-α基因启动子区甲基化状态,颈部血管超声测CIMT。用Spearman秩相关分析。结果EH组ER-α基因启动子区甲基化比例显著高于对照组(52.9%vs 30.0%,P=0.013),且高血压1级、高血压2级和高血压3级患者ER-α基因启动子区甲基化比例比较,差异有统计学意义(29.2%vs 52.2%vs 78.3%,P=0.003)。EH组血浆Hcy水平明显高于对照组[(13.34±4.22)μmol/L vs (10.34±3.10)μmol/L,P=0.000]。EH组中合并HHcy患者ER-α基因启动子区甲基化比例明显高于Hcy正常者(100.0%vs 29.8%,P=0.00)。EH组ER-α基因启动子区甲基化水平与血浆Hcy水平和CIMT呈正相关(r=0.722,r=0.718,P=0.00)。结论EH患者ER-α基因启动子区呈异常高甲基化,且其甲基化水平与血浆Hcy水平和CIMT具有显著的相关性。Objective To analyze the relationship of methylation status of ER-αgene with plasma Hcy level and carotid IMT in EH patients.Methods Seventy EH patients served as an EH group and 50 healthy persons served as a control group.Their plasma Hcy level was measured.The methylation status of ER-αgene was detected by nMSP.The carotid IMT was measured by carotid ultrasonography.The results were statistically analyzed.Results The methylation ratio of ER-αgene was significantly higher in EH group than in healthy controls(52.9%vs 30.0%,P=0.013),and there are statistically significant differences in methylation ratio of ER-αgene in patients with grades 1-3 hypertension(29.2%vs 52.2%vs 78.3%,P=0.003).The plasma Hcy level was obviously higher in EH group than in controls group(13.34±4.22μmol/L vs 10.34±3.10μmol/L,P=0.000).The methylation ratio of ER-αgene was significantly higher in patients with HHcy than in those with normal Hcy(100.0%vs 29.8%,P=0.00)and positively correlated with plasma Hcy level and carotid IMT(r=0.722,r=0.718,P=0.00).Conclusion ER-αgene promoter is abnormally hypermethylated in EH patients,which is positively related with plasma Hcy level and carotid IMT.

关 键 词：高半胱氨酸 雌激素受体Α 甲基化 高血压 

分 类 号：R544.11[医药卫生—心血管疾病]