益气养阴疏肝合剂通过Fas/FasL途径治疗大鼠桥本氏甲状腺炎  

Qi-replenishing,Yin-nourishing and Liver-soothing Mixture Treating Hashimoto’s Thyroiditis through Fas/FasL Pathway

作　　者：李婵 孙勤国 LI Chan;SUN Qin-Guo(Dept,of Traditional Chinese Medicine,Tongren Hospital of Wuhan University(Wuhan Third Hospital),Wuhan 430062 Hubei,China)

机构地区：[1]武汉大学同仁医院(武汉市第三医院)中医科

出　　处：《广州中医药大学学报》2020年第1期134-139,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：武汉市临床医学科研项目（编号：WZ14C02）

摘　　要：【目的】观察益气养阴疏肝合剂对桥本氏甲状腺炎(HT)大鼠甲状腺功能的影响。【方法】将60只SD雌性大鼠随机分为正常组、模型组、中成药组、中药合剂组,每组15只。模型组、中成药组、中药合剂组应用高碘诱发制备大鼠实验性自身免疫性甲状腺炎模型。造模后,中药合剂组给予益气养阴疏肝合剂灌胃;中成药组给予雷公藤多苷片悬浊液灌胃;模型组及正常组灌服等量蒸馏水。采用酶联免疫吸附法(ELISA)检测血清甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)、肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-10、IL-6、IL-12水平的变化,免疫组化法检测甲状腺组织Fas、FasL的表达。【结果】与正常组比较,模型组血清TGAb、TPOAb、TNF-α、IL-6及甲状腺组织Fas、FasL水平均显著升高,血清IL-10、IL-12水平均显著降低(P<0.01);与模型组比较,中药合剂组及中成药组血清TGAb、TPOAb、TNF-α、IL-6及甲状腺组织Fas、FasL水平均显著降低,血清IL-10、IL-12水平均显著升高(P<0.01),而2个治疗组组间比较,差异无统计学意义(P>0.05)。【结论】益气养阴疏肝合剂可缓解大鼠桥本氏甲状腺炎,其机制可能与调节Th1/Th2平衡,降低TGAb、TPOA表达,提高自身免疫水平有关。Objective To observe the effect of Qi-replenishing,Yin-nourishing and Liver-soothing Mixture(QYLM)on the thyroid function in Hashimoto’s thyroiditis rats.Methods Sixty SD female rats were randomly divided into normal group,model group,Chinese patent medicine group and Chinese medicine mixture group,15 rats in each group.Rats in the model group,Chinese patent medicine group and Chinese medicine mixture group were induced into thyroiditis model by high iodine.After modeling,the Chinese medicine mixture group was given intragastric administration of QYLM,the Chinese patent medicine group was given intragastric administration of suspension of tripterygium wilfordii polyglycoside tablets,and the normal group and model group were given intragastric administration of the same volume of distilled water.The levels of serum thyroglobulin antibody(TGAb),thyroid peroxidase antibody(TPOAb),tumor necrosis factorα(TNF-α),and interleukin(IL)-10,IL-6 and IL-12 were detected by enzyme linked immunosorbent assay(ELISA),and the expression levels of Fas and FasL in thyroid tissue were detected by immunohistochemistry.Results Compared with the normal group,the levels of TGAb,TPOAb,TNF-αand IL-6 in serum,and Fas and FasL in thyroid tissue were increased in the model group,and the levels of IL-10 and IL-12 in serum were decreased.Compared with the model group,the levels of TGAb,TPOAb,TNF-αand IL-6 in serum,and Fas and FasL in thyroid tissue were decreased in the Chinese medicine mixture group and Chinese patent medicine group,and the levels of IL-10 and IL-12 in serum were increased,the difference being insignificant between the two treatment groups(P>0.05).Conclusion QYLM can relieve rat Hashimoto’s thyroiditis,its mechanism is related with regulating the balance of Th1/Th2,decreasing TGAb and TPOAb expression to enhance autoimmune.

关 键 词：益气养阴疏肝合剂 桥本氏甲状腺炎 TGAB 细胞因子 FAS/FASL 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]