扶正抗癌方通过调控Bcl-2家族蛋白促肺癌细胞凋亡  

Supporting Healthy Qi and Anti-cancer Recipe Promotes Lung Cancer Cell Apoptosis through Modulating Bcl-2 Family Proteins

作　　者：李文娟 王苏美 LI Wen-Juan;WANG Su-Mei(Basic Medicine College,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Guangzhou Kangda Vocational Technical College,Guangzhou 511356 Guangdong,China;Dept,of Oncology,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine/Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120 Guangdong,China)

机构地区：[1]广州中医药大学基础医学院,广东广州510006 [2]广州康大职业技术学院,广东广州511356 [3]广州中医药大学第二附属医院/广东省中医院肿瘤科,广东广州510120

出　　处：《广州中医药大学学报》2020年第2期306-311,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目（编号：81903991,81974543）;广东省自然科学基金资助项目（编号：2017A030310326）

摘　　要：【目的】探讨中药复方扶正抗癌方促非小细胞肺癌(NSCLC)细胞凋亡的机制。【方法】以高效液相色谱(HPLC)法检测扶正抗癌方的稳定性。以人NSCLC细胞株H1650、A549为研究对象,采用流式细胞术检测不同剂量(0、0.5、1.0、1.5 mg/mL)扶正抗癌方处理的H1650、A549细胞的凋亡情况,蛋白免疫印迹(Western Blot)法检测不同剂量(0、0.5、1.0、1.5 mg/mL)扶正抗癌方处理的H1650、A549细胞中抗凋亡基因Bcl-2、Mcl-1的蛋白表达水平。【结果】扶正抗癌方可明显增加H1650、A549细胞凋亡率,抑制Bcl-2、Mcl-1的蛋白表达水平,均呈剂量依赖性。【结论】扶正抗癌方可通过调控Bcl-2家族蛋白表达促进非小细胞肺癌细胞凋亡。Objective To explore the mechanism of Supporting Healthy Qi and Anti-cancer Recipe for promoting the apoptosis of non-small cell lung cancer(NSCLC)cells.Methods The stability of Supporting Healthy Qi and Anti-cancer Recipe was analyzed by high performance liquid chromatography(HPLC).With human NSCLC cell lines H1650 and A549 as subjects,the apoptosis of H1650 and A549 cells treated with different dosages of 0,0.5,1.0,1.5 mg/mL of Supporting Healthy Qi and Anti-cancer Recipe was detected by flow cytometry.The protein expression levels of anti-apoptosis genes Bcl-2 and Mcl-1 in H1650 and A549 cells treated with different dosages of 0,0.5,1.0,1.5 mg/mL of Supporting Healthy Qi and Anti-cancer Recipe were detected by Western blotting method.Results Supporting Healthy Qi and Anti-cancer Recipe obviously increased the apoptosis rate of H1650 and A549 cells,and inhibited the protein expression levels of anti-apoptosis genes Bcl-2 and Mcl-1 in H1650 and A549 cells in a dose-dependent manner.Conclusion Supporting Healthy Qi and Anti-cancer Recipe promotes the apoptosis of NSCLC cells by regulating the expression of Bcl-2 family proteins.

关 键 词：扶正抗癌方 肺癌 凋亡 BCL-2家族 H1650 A549 

分 类 号：R285.5[医药卫生—中药学]