基于纳米孔测序技术的 PNA/λDNA测序精确度研究  

Research on PNA /λ DNA sequencing accuracy based on nanopore sequencing technology

作　　者：罗帆 熊珊珊 Luo Fan;Xiong Shanshan(School of Mechanical Engineering,Southeast University,Jiangsu Nanjing,211189,China)

机构地区：[1]东南大学机械工程学院

出　　处：《机械设计与制造工程》2020年第1期35-38,共4页Machine Design and Manufacturing Engineering

摘　　要：通过字符串匹配算法模拟PNA探针与λDNA匹配过程。在考虑PNA探针的碱基数目以及芯片厚度的情况下,模拟了3merPNA探针和5merPNA探针与λDNA的匹配过程,以读取λDNA碱基序列信息。实验结果表明,基于单分子纳米孔的第三代测序技术读取λDNA碱基可以达到较高的精度,为下一步基因测序实验奠定了理论基础。Based on string matching algorithm it introduces the simulation of the match process between PNA andλDNA.In consideration of the number of bases of the probe and the thickness of the chip,it simulates the 3mer and 5mer PNA probes matched with λDNA to read theλDNA base sequence information.The experimental data prove that the third-generation sequencing technology based on single-molecule nanopore can achieve high precision by reading DNA bases,which provides a theoretical basis for the next step of gene sequencing experiments.

关 键 词：字符串匹配算法 PNA DNA测序 

分 类 号：TP301.6[自动化与计算机技术—计算机系统结构]