两株植物内生菌促生和诱导辣椒植株对南方根结线虫抗性的比较分析  

Comparative Analysis of Growth Promotion and Resistance Induction of Pepper Plants to Meloidogyne incognita by Two Endophytes

作　　者：杨亚茹 茆少星 闫淑珍[1] 陆长梅[1] Yang Yaru;Mao Shaoxing;Yan Shuzhen;Lu Changmei(School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院

出　　处：《南京师大学报（自然科学版）》2019年第4期77-84,共8页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省自然科学基金项目(BK2012848)

摘　　要：本文以两株产ACC脱氨酶的内生菌蜡状芽孢杆菌SZ5和荧光假单胞菌DLJ1为材料,探讨其在辣椒促生和预防线虫病害方面的作用.结果显示,虽然两菌剂处理不能促进种子萌发,但对幼苗生长有短暂促进作用;促生作用消失后,植株体内次生代谢物质含量和抗氧化酶活性仍高于对照,DLJ1组高于SZ5组.挑战接种南方根结线虫后,接菌组植物体内的POD等酶活性以及总黄酮等物质含量的提高幅度高于对照.菌剂处理降低了植株染病率和染病程度,提高了辣椒产量和品质;萌发期一次处理效果优于后期的多次处理,DLJ1的处理效果优于SZ5.总之,DLJ1在辣椒种子萌发期一次接菌就可以有效促进辣椒生长,提高植株对南方根结线虫的抗性,提高辣椒产量.该作用可能与萌发期菌株的有效定植、生长期的抗氧化能力的维持与高效反应,以及有效降低乙烯产量有关.Meloidogyne incognita is the most common soil borne disease of pepper. Two endophytes,SZ5( Bacillus cereus)and DLJ1( Pseudomonas fluorescens biovar I),which can produce ACC deaminase,were used as materials to study their effects on the growth and resistance of pepper plants to M. incognita. Results showed that although the two endophytes could not promote seed germination,they could promote seedling growth for a short time. After the short-term effect disappeared,the contents of secondary metabolites and the activities of antioxidant enzymes of plants in test groups were still higher than those in the control,and those in the DLJ1 group were higher than those in the SZ5 group. After challenge inoculation with M. incognita,the activities of POD etc. and the contents of total flavonoids in the test plants were higher than those in the control. Treatment with DLJ1 or SZ5 reduced the infection rate and degree of M. incognita in plants,increased the yield and quality of pepper fruits. The effects of once inoculation at germination stage were better than those of multiple inoculations at transplantation and later,those of DLJ1 inoculation was better than those of SZ5 inoculation.In a word,once DLJ1 inoculation at germination stage can effectively promote the growth of pepper plants,increase its resistance to M. incognita and increase the yield and quality of its fruits. These effects may be related to the effective colonization of strains at germination,the maintenance of antioxidant capacity and efficient response of plants to stress,as well as the effective reduction of ethylene production in plants at growth stage.

关 键 词：植物内生菌 辣椒 南方根结线虫 促生 抗性 产量 

分 类 号：S43[农业科学—农业昆虫与害虫防治]