黄芪丹参配伍提取物经VEGF、Ang1/Tie2通路对心肌梗死大鼠血管新生的病理影响  

Pathological Effect of Astragalus-salvia-miltiorrhiza Extract on Angiogenesis of MI Rats via VEGF and Ang1/Tie2 Pathway

作　　者：张瓅方[1] 李梦华[2] 刘暖[1] 杨雷[1] 毛秉豫[1] ZHANG Li-fang;LI Meng-hua;LIU Nuan;YANG Lei;MAO Bing-yu(Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technolog,Nanyang 473004,China;Nanyang Medical College,Nanyang 473061,China)

机构地区：[1]南阳理工学院河南省张仲景方药与免疫调节重点实验室,南阳473004 [2]南阳医学高等专科学校,南阳473061

出　　处：《科学技术与工程》2020年第1期104-108,共5页Science Technology and Engineering

基　　金：国家自然科学基金(81473438);河南省高等学校重点科研项目(19A360031);南阳市科技攻关项目(KJGG2018077)

摘　　要：为观察黄芪、丹参配伍提取物对心肌梗死大鼠VEGF(vascular endothelial growth factor)、血管生成素Ang1及其受体酪氨酸激酶Tie2信号通路的表达影响,对心肌梗死大鼠模型复制成功后分为假手术对照组、模型组、黄芪、丹参配伍提取物低、中、高剂量组,分别予以对应药物灌胃4周。观察大鼠一般生活状态,并分别采用HE染色和免疫组织化学染色测试大鼠心肌组织病理形态结构和VEGF、Ang1、Tie2的蛋白表达。结果表明:模型组心肌结构紊乱伴有炎性侵润,VEGF、Ang1和Tie2蛋白表达较假手术对照组有所增加(P<0.05);低、中、高剂量组的这些表达随着药物浓度梯度增加,心肌结构逐渐规整,内皮细胞完整、新生血管增多,与模型组比较差异显著(P<0.05或P<0.01)。可见黄芪、丹参配伍提取物可以上调心肌梗死大鼠VEGF、Ang1和Tie2的表达,促进血管新生。To observe the effects of extracts of Astragalus and Salvia miltiorrhiza on the expression of vascular endothelial growth factor(VEGF),angiopoietin Ang1,and its receptor tyrosine kinase Tie2 signaling pathway in rats with myocardial infarction,the rat models of myocardial infarction were successfully divided into sham-operated control group and model group and treated with the extract of Astragalus and Salvia miltiorrhiza in low,medium,and high dosages.The corresponding drugs were administered for 4 weeks.General living conditions of rats were observed,and the pathological structure of myocardium and the expressions of VEGF,Ang1,and Tie2 were measured by HE staining and immunohistochemical staining.The result shows that myocardial structural disorder was associated with inflammatory infiltration in the model group.The expression of VEGF,Ang1,and Tie2 protein was higher than that of the sham-operated control group(P<0.05),and the expressions of the low,medium,and high dose groups were increased with the concentration gradient of the drug.The structure was gradually regularized,the endothelial cells were intact,and the neovascularization was increased,which was significantly different from the model group(P<0.05 or P<0.01).Therefore,the extract of Astragalus-Salvia-miltiorrhiza can upregulate the expression of VEGF,Ang1,and Tie2 in rats with myocardial infarction and promote angiogenesis.

关 键 词：心肌梗死 血管新生 VEGF ANG1 TIE2 

分 类 号：R285.5[医药卫生—中药学]