糖通饮对2型糖尿病大鼠胰腺组织PI3K-AKT通路的影响  

The Effect of Tangtongyin Formula on the PI3K-AKT Signaling Pathway in the Pancreas of Type 2 Diabetic Rats

作　　者：马欢 高楠楠[1] 陈俞如 肖瑛[1,2] 潘艳伶 MA Huan;GAO Nannan;CHEN Yuru;XIAO Ying;PAN Yanling(Guizhou Medical University,Guiyang 550004,Guizhou China;The Affiliated Hospital of Guizhou Medical University,Guiyang 550025,Guizhou China;Key Laboratory of Pathogenesis and Drug Research of Common Chronic Diseases in Guizhou Province,Guiyang 550004,Guizhou China)

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州省常见慢性疾病发病机制及药物研究重点实验室,贵州贵阳550025 [3]贵州医科大学附院,贵州贵阳550004

出　　处：《贵州医科大学学报》2020年第1期50-55,共6页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目课题[黔科合LH字(2016)7248号];贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2016-006)

摘　　要：目的:观察糖通饮对2型糖尿病(T2DM)大鼠胰腺组织磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(AKT)信号通路的影响,探讨糖通饮对2型糖尿病大鼠胰腺组织的保护作用及其机制。方法:50只SPF级SD雄性大鼠,随机均分为对照组,模型组,糖通饮低、中及高剂量组;对照组给予正常饲料喂养,其余4组大鼠采用高脂饲料联合小剂量多次腹腔注射链脲佐菌素制备T2DM大鼠模型,造模成功的糖通饮低、中及高剂量组大鼠给予糖通饮(10、20及30 mg/kg)灌胃治疗8周,对照组和模型组大鼠给予同体积双蒸水处理;比较各组大鼠给药前,干预2、4及8周时的体质量,比较干预前及干预8周时各组大鼠的空腹血糖(FBG);干预8周时,逆转录实时荧光定量PCR法检测大鼠胰腺组织中AKT及胰岛素受体底物1(IRS-1)mRNA水平,免疫组织化学法检测大鼠胰腺组织中AKT及IRS-1蛋白表达。结果:干预前,干预2、4及8周时,模型组大鼠体质量较对照组显著降低(P<0.01);干预2周和4周时,糖通饮高剂量组大鼠体质量较模型组显著升高(P<0.05);干预8周时,糖通饮各剂量组大鼠体质量均较模型组显著升高(P<0.01),但各剂量组间比较,差异无统计学意义(P>0.05);干预8周时,与对照组比较,模型组大鼠FBG显著升高(P<0.01),胰腺组织AKT及IRS-1 mRNA和蛋白的表达均明显降低(P<0.01);与模型组比较,糖通饮各剂量组大鼠FBG明显降低(P<0.01),糖通饮高剂量组AKT及IRS-1 mRNA的表达水平显著升高(P<0.01),糖通饮高剂量组AKT和各剂量组IRS-1蛋白的表达也均显著增加(P<0.01)。结论:糖通饮可通过激活胰腺组织PI3K/AKT信号通路,改善T2DM大鼠胰岛素抵抗和降低血糖,其机制可能与影响PI3K/AKT信号通路中因子AKT的表达水平有关。Objective:To observe the effect of Tangtongyin formula on PI3K-AKT pathway in pancreas tissues of rats with Type 2 diabetes mellitus(T2DM),and to explore the protective effect and mechanism of Tangtongyin formula on pancreas tissues of rats with T2DM.Methods:50 SPF SD male rats were randomly divided into the control group,the model group and the low-dose,medium-dose and high-dose Tangtongyin groups,with 10 rats in each group.The control group took normal feed,and the other 4 groups with high fat feed combined with small dose of urea with intraperitoneal injection of multiple chain rhzomorph preparation of T2DM rat model;Successful medel groups with Tangtongyin formula low,medium and high doses received gastric gavage of Tangtongyin formula(10,20 and 30 mg/kg respectively)for 8 weeks;The control group and model group were given with double steaming water volume,and the comparison of body quality among the groups before intervention,and of 2-,4-and 8-week intervention,and the comparison of fasting blood glucose(FBG),mRNA levels of AKT and insulin receptor substrate 1(IRS-1)in the rat pancreas were made by real-time fluorescence quantitative PCR intervention for 8 weeks;The protein expressions of AKT and IRS-1 in the rat pancreas after intervention for 8 weeks were detected by immunohistochemistry.After 8 weeks,body weight,FBG were measured.The expression of AKT and IRS-1 in rat pancreas was detected by real-time fluorescence quantitative PCR,and the expression of AKT and IRS-1 protein in rat pancreas was detected by immunohistochemistry.Results:Before intervention,and after intervention for 2,4 and 8 weeks the body mass of rats in the model group was significantly lower than that in the control group(P<0.01).After intervention for 2 weeks and 4 weeks,the body mass of rats in the high-dose group was significantly higher than that in the model group(P<0.05).After intervention for 8 weeks,the body mass of rats in each Tangtongyin dose group was significantly higher than that in the model group(P<0.01),but the differen

关 键 词：2型糖尿病 糖通饮 胰腺组织 糖代谢 胰岛素受体底物1 磷脂酰肌醇3激酶 蛋白激酶B 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]