肥胖大鼠袖状胃切除术后肠源性脂多糖的变化及其与体重变化的相关性研究  

Change of enterogenous lipopolysaccharide and its correlation analysis with weight change after sleeve gastrectomy in obese rats

作　　者：黎熊 刘斌 李佳俊 刘伟 Li Xiong;Liu Bin;Li Jiajun;Liu Wei(Department of General Surgery,the Second Affiliated Hospital of Chengdu Medical College,Nuclear industry 416 hospital,Chengdu 610000,China)

机构地区：[1]成都医学院第二附属医院·核工业四一六医院普外科

出　　处：《中华肥胖与代谢病电子杂志》2019年第3期128-136,共9页Chinese Journal Of Obesity and Metabolic Diseases：Electronic Edition

基　　金：四川省卫计委科研项目基金(18PJ489)

摘　　要：目的通过检测肥胖大鼠在袖状胃切除术前后体内脂多糖的变化及分布探究脂多糖与大鼠术后体重变化的相关性。方法 (1)建立肥胖大鼠模型成功后测量大鼠体重、体长、糖耐量及胰岛素抵抗水平并随机分组。手术组行袖状胃切除术,术后每周随机处死5只大鼠。假手术组行剖腹探查术,处死及分组同手术组。(2)术后每2日测量两组大鼠体重。(3)取两组大鼠术前及术后粪便行革兰染色,计算菌群比例。(4)手术前后定时抽取两组大鼠尾静脉血液,检测其血浆LPS水平。(5)处死大鼠时,取两组大鼠肝脏、肠系膜、肾周脂肪、附睾脂肪等组织检测其LPS水平。(6)术后对各组大鼠行糖耐量试验及胰岛素抵抗水平检测。结果 (1)术后手术组大鼠体重进行性下降,而假手术组大鼠体重下降后回升。(2)与假手术组相比,手术组大鼠术后粪便革兰阳性菌数量增多,革兰阴性菌减少。(3)术后手术组大鼠血清LPS水平下降,而假手术组大鼠血清LPS水平下降后回升并超过术前水平。且两组大鼠血清LPS水平与取血当时的体重水平有显著的直线相关性(P=0.0004)。(4)与假手术组相比,手术组大鼠腹腔脂肪中LPS水平随术后时间降低。(5)术后手术组大鼠糖耐量水平及HOMA-IR指数降低,而假手术组升高。结论 (1)肥胖大鼠袖状胃切除术后,大鼠体内LPS水平的变化与其体重变化存在显著相关性;(2)肥胖大鼠袖状胃切除术后,大鼠糖耐量及胰岛素敏感性得到改善。Objective This study aims to explore the correlation by detecting the metabolic changes and internal distribution of the LPS contained in rat during preoperative and postoperative period of sleeve gastrectomy.Methods (1) After successful obese rats modeling did,the weight,body length,glucose tolerance and insulin resistance level were measured and grouping them randomized.Rats in surgical group were performed sleeve gastrectomy.Then execute 5 rats per week.Rats in sham operation group were performed exploratory Laparotomy,whose grouping and execution coincides with surgical group.(2) Rats weight of surgical group and sham operation group were measured once for two days after operation.(3) Faeces of rats from two groups were selected both preoperatively and postoperatively,which were used to analysis the proportion of the gram-positive bacterium and gram-negative bacterium.(4) Rats’ blood in two groups were collected regularly both before and post operation to measure serum levels of LPS.(5) In surgical group and sham operation group,tissues of liver,mesentery fat,perirenal fat and epididymal fat were picked and grinded for tissue homogenate when rats were executed to quantify the LPS contained in these tissues.6) Glucose tolerance tests and insulin resistance level tests were performed in each group both preoperatively and postoperatively.Results (1) The weight of rats in sham operation group rebound gradually after decreased,even more heavier than preoperative.Oppositely,the weight of rats in surgical group decreased progressively and then stabilized.(2) the proportion of gram-positive bacteria of surgical group were increased compared with sham operation group.On the contrary,the proportion of gram-negative bacteria of surgical group were reduced.(3) The rats’ serum level of LPS in operation group declined obviously after operation,however,the rats’ serum level of LPS in sham operation group climbed up higher than preoperative levels.Also,serum levels of LPS in the two groups have significant linear corre

关 键 词：袖状胃切除术 肠源性脂多糖 肥胖大鼠模型 

分 类 号：R589.2[医药卫生—内分泌] R-332[医药卫生—内科学]